CRISPR Cas9-gRNA Service
The CRISPR/Cas9 or Cpf1 system uses a genome editing technology to modify genes using Nuclease (Cas9 or Cpf1) and gRNA (guide RNA) complexes.
Bioneer’s AccuCRISPR™-Cas9/Cpf1 gRNA offers customized RNA synthesis service. Simply enter the sequences known from your past experiences or a research paper in the order manual. Otherwise, please use the CRISPR-gRNA Custom Design service to clarify the required sequence beforehand. See “Ordering Information” section for more details.
The ordered gRNA can be used in the form of ribonucleoprotein (RNP) with Nuclease (Cas9 or Cpf1). Since the RNP form can be rapidly degraded by endogenous protease or RNase, It has the advantage of lower off-target.
Bioneer offers CRISPR-gRNA service based on a long-term oligo synthesis technology. All the gRNAs have passed RNA quality control (QC) through synthesis in our state-of-the-art clean room facility and purification using MALDI-TOF mass spectrometry.
The gRNA of CRISPR-Cas9 consists of a crRNA with target-specific sequence and a tracrRNA with Cas9 nuclease binding sequence. Bioneer provides both crRNA and tracrRNA, to easily construct in a crRNA+tracrRNA complex form to be used as a RNP (ribonucleoprotein) in the CRISPR system.
Features and Benefits
Ready-to-use guide RNAs compatible with nuclease without the need of cloning process
Qualified products through the synthesis in a sterile room
Advanced RNA oligo synthesis technology
Comprehensive gRNA design compatible with various CRISPR nuclease
Figure1. In-vitro digestion of pBHA vector (plasmid & PCR product) using Cas9 nuclease and Bioneer’s gRNA(tracrRNA : crRNA duplex form). Products were resolved on a 1% agarose TAE gel.
- Plasmid & PCR product size: 2000bp
- cleaved PCR fragment size: 1200bp+800bp