CRISPR-Cas9 tracrRNA works as a duplex form with crRNA.
As its sequence is already fixed, simply select the quantity and scale during your order to use your crRNA with CRISPR system.
While you can order AccuCRISPR™-Cas9 tracrRNA under "CRISPR Cas9-gRNA Service" along with crRNA, if you need additional tracrRNA, simply place order in this page.
The ordered gRNA can be used in the form of ribonucleoprotein (RNP) with Nuclease (Cas9 or Cpf1). Since the RNP form can be rapidly degraded by endogenous protease or RNase, It has the advantage of lower off-target.
Bioneer offers CRISPR-gRNA service based on a long-term oligo synthesis technology. All the gRNAs have passed RNA quality control (QC) through synthesis in our state-of-the-art clean room facility and purification using MALDI-TOF mass spectrometry.
The gRNA of CRISPR-Cas9 consists of a crRNA with target-specific sequence and a tracrRNA with Cas9 nuclease binding sequence. Bioneer provides both crRNA and tracrRNA, to easily construct in a crRNA+tracrRNA complex form to be used as a RNP (ribonucleoprotein) in the CRISPR system.
Ready-to-use guide RNAs compatible with nuclease without the need of cloning process
Qualified products through the synthesis in a sterile room
Advanced RNA oligo synthesis technology
Comprehensive gRNA design compatible with various CRISPR nuclease