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AccuPower® Plus DualStar™ qPCR PreMix (with UDG), ABI7500 96-well plate (96 T, 50 μl)
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AccuPower® Plus DualStar™ qPCR PreMix (with UDG) can accurately quantify the target gene of interest in a in various kinds of samples. Hotstart Taq DNA Polymerase is used to solely amplify the gene of interest, with excellent sensitivity capable of amplifying template DNA even present in a trace amount, with no carryover contamination through the application of DNA glycosylase system. Moreover, this product is compatible with comprehensive types of devices, including Exicycler™ 96 and those from other companies, by providing optimized tube and plates, along with a 2X master mix type.
※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Features and Benefits
- Prevention of Carryover Contamination
Minimized false positives through the application of uracil DNA glycosylase system by preventing carryover contamination
- Wide Range
A wide range of 8 logs up to 10 ~ 108 copies.
- Specificity
Optimized amplification of target gene using Hotstart Taq DNA polymerase
- Comprehensiveness
Effective quantitative PCR results from a gene regardless of its type, including DNA, cDNA and high GC template.
- Conveniance
Simplified procedure as all reactants essential for real-time PCR included in each tubes to readily start by adding template DNA, probe & primer for target gene, and D.W.
- Stability
Enhanced stability being more stable the solution type products by including a stabilizer in the Real-Time PCR reaction mixture
- Reproducibility
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables through mass production under ISO 9001 quality system
Application
• Gene expression profiling
• Target DNA quantification
• Microbial detection
• Viral/bacterial pathogen load determination
• Probe-based real-time PCR primer pair performance evaluation
Experimental data
![figure1](/images/products/RP-accupower-PlusDualStar_UDG_TS1.jpg)
Figure. Comparison of sensitivity between AccuPower® Plus DualStar™ qPCR PreMix (K-6600) and AccuPower® Plus DualStar™ qPCR PreMix (with UDG) (K-6605).