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AccuPower® 2X GreenStar™ qPCR MasterMix (-ROX Dye) (2.5 ml)
AccuPower® 2X GreenStar™ qPCR Master Mix is a Real-Time PCR product with improved reaction specificity and PCR amplification efficiency by applying dsDNA binding dye and Top Antibody HotStart.
※This product is shipped in dry ice.
Features and Benefits
- Specificity
Minimized experimental failures caused by products from non-specific amplifications and increased efficiency of even a small amount of template DNA using Hotstart Top DNA polymerase
- Easy-of-use
Present in 2X master mix type without the need of dsDNA binding dye to minimize the error caused by the solution mixing
- Stability
Stabilizer included in the Real-Time PCR reaction mixture for long-term storage in a freezer and maintance of its performance activity
- Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables
Application
• Real-time quantification of DNA and cDNA targets
• Gene expression profiling
• Microbial & Viral pathogen detection
Experimental data
Figure. Highly reproducible Ct values
Amplification of an 90 bp target gene was detected using serially diluted LP(Legionella Pneumoniae) genomic DNA (106~101copies) with AccuPower® 2X Greenstar™ qPCR master mix. As shown in Figure, highly reproducible Ct values were achieved within each Lot. set of triplicates