AccuPower® 2X GreenStar™ qPCR MasterMix (-ROX Dye) (2.5 ml)

AccuPower^® 2X GreenStar™ qPCR Master Mix is a Real-Time PCR product with improved reaction specificity and PCR amplification efficiency by applying dsDNA binding dye and Top Antibody HotStart.

※This product is shipped in dry ice.

$151.00

Cat. No.

K-6253

Product Overview

Features and Benefits

Specificity
Minimized experimental failures caused by products from non-specific amplifications and increased efficiency of even a small amount of template DNA using Hotstart Top DNA polymerase
Easy-of-use
Present in 2X master mix type without the need of dsDNA binding dye to minimize the error caused by the solution mixing
Stability
Stabilizer included in the Real-Time PCR reaction mixture for long-term storage in a freezer and maintance of its performance activity
Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables

Application

• Real-time quantification of DNA and cDNA targets

• Gene expression profiling

• Microbial & Viral pathogen detection

Experimental data

Figure. Highly reproducible Ct values
Amplification of an 90 bp target gene was detected using serially diluted LP(Legionella Pneumoniae) genomic DNA (10⁶~10¹copies) with AccuPower^® 2X Greenstar™ qPCR master mix. As shown in Figure, highly reproducible Ct values were achieved within each Lot. set of triplicates

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
3' – A Overhang	Yes

▶ Experimental data

Equipment Compatibility

• Using a Bioneer Exicycler™ 96

- The annealing temperature can be set to 55~65°C, depending on the primer Tm value.

- The annealing time should be set for 5~20 seconds. Longer annealing time results in increased efficiency, and a shorter time decreases non-specific amplification.

• Using a ABI 7500

• Using a Bio-Rad

Figure 1. Comparison of the specificity of the intercalating dye-based real-time PCR products. Amplification of a 90 bp target gene was detected using serially diluted LP (Legionella Pneumoniae) genomic DNA (10ⁿdilution;10⁵~10¹copies). As shown in the Figure 1, very small amount of primer dimers was observed in AccuPower^®2X GreenStar™ qPCR Master mix compared with other kits.

Figure 2. Gene expression analysis AccuTarget ™ Validated Real-Time PCR Primer Library for Human is designed by Bioneer’s bioinformatics tool and targeting for human genome. cDNA was synthesized using human PTGS2 target primer of those and human total RNA identically quantified from Hela cell and blood cell with AccuPower^® CycleScript RT PreMix (Cat. No. K-2044, Bioneer). Gene analysis was carried out both Hela cell and blood cell by operating real-time PCR reaction (95°C 10 min, 1 cycle and 95°C 10 sec, 58°C 25 sec, 72°C 30 sec, 41 cycles) using the cDNA, AccuPower^® GreenStar™ qPCR Master Mix and Exicycler™ 96 Real-Time Thermal Block (Cat. No. A-2060, Bioneer).

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description	Option			Price
K-6251	AccuPower^® 2X Greenstar™ qPCR Master Mix	100 Rxn	50 μL reaction	with 80X ROX Dye	$K-6251
K-6253		100 Rxn	50 μL reaction		$K-6253
K-6252		200 Rxn	50 μL reaction	with 80X ROX Dye	$K-6252
K-6254		200 Rxn	50 μL reaction		$K-6254