▶ Specifications
| Scale |
Mini |
Midi |
| Expected RNA yield |
Up to 100 μg |
Up to 500 μg |
| Minimum elution volume |
50 µl |
500 µl |
| Preparation time |
~ 10 min |
~ 15 min |
| Sample type |
Starting amount |
RNA Yield |
Expected purity* |
| Cultured cells |
104-108 cells |
15 - 20 μg |
A260/280 > 2.0
A260/230 > 1.7 |
| Liver |
25 - 50 mg |
10 - 60 μg |
| Spleen |
100 mg |
30 - 60 μg |
| Plant tissue |
100 mg |
70 - 80 μg |
*Differences in the quantified values may occur depending on the types of samples.
▶ Components
| Components |
K-3613G* |
| RD Buffer (RNA Binding) |
30 ml x 2 ea |
| RWMB1 Buffer (1st washing) |
50 ml x 1 ea |
| RWB2 Buffer (2nd washing) |
30 ml x 1 ea |
| WE Buffer (3rd washing) |
120 ml x 1 ea |
| ER Buffer (Elution) |
20 ml x 1 ea |
| Magnetic Nanobead |
11 ml x 1 ea |
| User's Guide |
1 ea |
*mini-100 rxn, midi-10 rxn
▶ Experimental data
| Prep kit |
No. |
Conc. (ng/μl) |
260/280 |
260/230 |
| ExiPrep™ 96 |
1 |
161.363 |
2.034 |
2.034 |
| 2 |
224.668 |
2.025 |
0.995 |
| 3 |
244.232 |
2.050 |
1.992 |
| 4 |
262.524 |
2.052 |
2.109 |
| 5 |
254.540 |
2.043 |
2.069 |
| 6 |
245.293 |
2.057 |
1.911 |
| 7 |
188.215 |
2.035 |
1.862 |
| 8 |
250.754 |
2.028 |
1.980 |
Figure 1. Cultured cell RNA extraction using MagListo™ 5M Universal RNA Extraction Kit on ExiPrep™96 Lite.
RNA extraction from 1 x 106 cells of Hela. DNase was not treated. (average of concentration is 228.9 ng/μl, average A260/280 is 2.04).
| Prep kit |
No. |
Conc. (ng/μl) |
260/280 |
260/230 |
| ExiPrep™96 |
1 |
704.166 |
2.160 |
2.397 |
| 2 |
586.781 |
2.204 |
2.393 |
| 3 |
823.105 |
2.174 |
2.429 |
| 4 |
629.669 |
2.190 |
2.328 |
| 5 |
700.043 |
2.171 |
2.337 |
| 6 |
707.035 |
2.175 |
2.296 |
| 7 |
609.375 |
2.196 |
2.217 |
| 8 |
516.164 |
2.176 |
2.345 |
Figure 2. Plant tissue RNA extraction using MagListo™ 5M Universal RNA Extraction Kit on ExiPrep™96 Lite.
RNA extraction from ~ 100 mg of Brassica oleracea. DNase was not treated. (average of concentration is 659.5 ng/μl, average A260/280 is 2.18).
| Prep kit |
No. |
Conc. (ng/μl) |
260/280 |
260/230 |
| ExiPrep™96 |
1 |
774.547 |
2.006 |
2.333 |
| 2 |
690.183 |
2.031 |
2.409 |
| 3 |
694.402 |
2.068 |
1.912 |
| 4 |
706.923 |
2.022 |
2.392 |
| 5 |
759.792 |
2.025 |
2.373 |
| 6 |
670.353 |
2.016 |
2.404 |
| 7 |
802.516 |
2.031 |
2.371 |
| 8 |
884.264 |
2.055 |
2.402 |
Figure 3. Animal tissue RNA extraction using MagListo™ 5M Universal RNA Extraction Kit on ExiPrep™96 Lite.
RNA extraction from ~ 20 mg of mouse kidney. DNase was not treated. (average of concentration is 747.9 ng/μl, average A260/280 is 2.03).
| Prep kit |
Conc. (ng/μl) |
260/280 |
260/230 |
| MagListo™ |
249.9 |
2.08 |
2.19 |
| 282.8 |
2.05 |
2.24 |
| ExiPrep™ 96 |
188.2 |
2.04 |
1.86 |
| 250.8 |
2.03 |
1.98 |
| Company Q |
228.6 |
2.07 |
2.17 |
| 205.2 |
2.08 |
1.54 |
Figure 4. Cultured cell RNA extraction using MagListo™ 5M Universal RNA Extraction Kit.
RNA extraction from 1 x 106 of Hela cell. DNase was not treated.
1: MagListo (Bioneer), 2: ExiPrep 96 Lite(Bioneer, Automated system), 3: Company Q
| Prep kit |
Conc. (ng/μl) |
260/280 |
260/230 |
| MagListo™ |
753.8 |
2.20 |
2.23 |
| 703.8 |
2.15 |
1.76 |
| ExiPrep™ 96 |
704.2 |
2.16 |
2.40 |
| 823.1 |
2.17 |
2.43 |
| Company Q |
635.4 |
2.19 |
2.00 |
| 642.8 |
2.18 |
1.01 |
Figure 5. Plant RNA extraction using MagListo™ 5M Universal RNA Extraction Kit.
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: MagListo (Bioneer), 2: ExiPrep 96 Lite(Bioneer, Automated system), 3: Company Q
| Prep kit |
Conc. (ng/μl) |
260/280 |
260/230 |
| MagListo™ |
511.0 |
2.08 |
2.26 |
| 510.9 |
2.05 |
2.24 |
| ExiPrep™ 96 |
690.2 |
2.03 |
2.41 |
| 694.4 |
2.07 |
1.91 |
| Company Q |
461.7 |
2.12 |
1.89 |
| 490.1 |
2.10 |
2.01 |
Figure 6. Animal tissue RNA extraction using MagListo™ 5M Universal RNA Extraction Kit.
RNA extraction 20 mg of Mus musculus liver. ell. DNase was not treated.
1: MagListo (Bioneer), 2: ExiPrep 96 Lite(Bioneer, Automated system), 3: Company Q
