▶ Specifications
| Feature |
Specification |
| Starting culture voulme |
1 - 10 ml |
| Preparation time |
< 20 min |
| Elution volume |
50 - 100 µl |
| Expected DNA yield* |
Up to 20 µg |
| Expected DNA purity |
A260/280 > 1.8 |
* Differences in the quantified value may occur depending on the types of plasmids.
▶ Components
| Components |
K-3030
(200 reaction) |
K-3030-1
(50 reaction) |
| PA1 Buffer (Resuspension) |
60 ml X 1 ea |
15 ml X 1 ea |
| P2 Buffer (Lysis) |
60 ml X 1 ea |
15 ml X 1 ea |
| PA3 Buffer (Neutralization) |
80 ml X 1 ea |
20 ml X 1 ea |
| PB Buffer (Endonuclease A denaturation) |
75 ml X 1 ea |
20 ml X 1 ea |
| W2 Buffer (Washing) |
80 ml X 2 ea |
40 ml X 1 ea |
| EA Buffer (Elution) |
25 ml X 1 ea |
15 ml X 1 ea |
| BST Solution |
40 ml X 1 ea |
10 ml X 1 ea |
| RNase A powder, lyophilized |
6 mg X 1 ea |
1.5 mg X 1 ea |
| AccuPrep® Binding Column-II plus |
200 ea |
50 ea |
| Collection Tube (for filtration) |
200 ea |
50 ea |
| User's Guide |
1 ea |
1 ea |
▶ Procedure
▶ Experimental data
Figure 1. Improved yield.
AccuPrep® Plasmid Mini Extraction Kit shows high yields in all plasmid DNA of high & low copy number.
- Lane B; AccuPrep® Plasmid Mini Extraction Kit
- Lane 2, 3; Competitors
Figure 2. Restriction enzyme digestion test.
200 ng of extracted plasmid DNA (pBluescript SK(+)) were digested using XbaI. Each of the enzymes was able to recognize its binding site.
- Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No. D-1070, Bioneer)
- Lane 1; AccuPrep® Plasmid Mini Extraction Kit
- Lane 2, 3; Competitors
200 ng of extracted plasmid DNA (pBluescript SK(+)) were digested with various restriction enzymes. Each of the enzymes was able recognize its binding site.
- Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No., D-1070, Bioneer)
- K; Kpn I - X; Xho I
- SI; Sal I - H; Hind III
- E; EcoR I - P; Pst I
- Sm; Sma I - B; Bam HI
- S; Spe I - Xb; Xba I
- N; Not I - Sc; Sac I
