Overview
Plasmodium spp. is the causative organism of malaria, which is an epidemic that has a high risk and a mortality rate. Each year, it infects more than 200 million people worldwide. When bitten by an infected female mosquito, people may show various symptoms, such as headache, splenomegaly, jaundice, and anemia. Patients may also show signs of shiver and high fever of above 39°C. In the Republic of Korea, Plasmodium vivax is the major cause of malaria, hence polymerase chain reaction (PCR) specific to Plasmodium vivax is being used for an accurate diagnosis of the infectious agent.
Features and Benefits
- Nested PCR
Detect Plasmodium vivax with nested PCR consisting of rPLU PCR Kit and rVIV PCR Kit.
- Convenience & Reproducibility
All reactants necessary for PCR including primers are lyophilized in each PCR tube, providing reproducible results in a convenient way.
- Sensitivity
By applying the patented PyroHotStart(Enzyme-mediated HotStart) technology that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
- Stability
Included stabilizer in the PCR reaction mixture provides increased stability compared to solution-type products.
▶ Specifications
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Type
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PreMix
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Target
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Plasmodium vivax
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Instrument
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Thermal Cycler
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Specimen
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Environmental sample etc.
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Tests per kit
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96 tests
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▶ Experimental Data
Figure 1. Malaria detection experiment result.
AccuPower® rPLU/rVIV PCR Kit was used, and 5 µl PCR reaction products were electrophoresed on 1.5% TBE Agarose gel.
◈ Manual
• Manual_AccuPower® rPLU/rVIV PCR Kit
◈ MSDS
• MSDS_K-2940 AccuPower® rPLU/rVIV PCR Kit EN ver.0
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
