We use cookies to give you the best online experience. By using our website you agree to our use of cookies in accordance with our Cookie Policy
AccuPower® ProFi Taq PCR Master Mix (2X, 1ml)
![](https://eng.bioneer.com/media/catalog/product/cache/image/210x140/af097278c5db4767b0fe9bb92fe21690/_/k/_k-2264__4.jpg)
AccuPower® ProFi Taq PCR Master Mix is the Master Mix type of AccuPower® ProFi Taq PCR Premix products.
※ This product is shipped in dry ice
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Features and Benefits
- High Efficiency, High Sensitivity PCR Amplification
Guaranteed accurate PCR products with the excellent sensitivity and the best amplification efficiency
- Long Range PCR
Effective amplification of large genomic DNA fragments up to 20 kb of human DNA
- Convenience
Simplified procedure only requiring the addition of templates and primers with vaccum-dried components
- Stability
Enhanced stability allowing maintaince of stable enzymatic activities after long-term storage by including a stabilizer and dried in the PCR reaction mixture
- Reproducibility
Minimized deviation between lots through a mass production under ISO 9001 quality system, allowing reproducible results even for replicates tested repeatedly under same conditions and variables
Application
• Primer extension
• long-range amplification from genomic DNA
• High amplification efficiency
• Excellent performance on difficult templates
• Amplification of low-copy targets
• High yield and high sensitivity PCR
Experimental data
![accupower](/images/products/accupower_ProFi_taq_f01.gif)
Figure. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.
Target : human Insulin receptor gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human genomic DNA
Lane 2 : 1 ng of human genomic DNA
Lane 3 : 100 pg of human genomic DNA
Lane 4 : 10 pg of human genomic DNA