AccuPower® ProFi Taq PCR PreMix (480 T, 20 μl)

AccuPower® ProFi Taq PCR PreMix uses ProFi Taq DNA polymerase developed by Bioneer which allows accurate amplification of long template DNA with high fidelity and efficiency. As the components necessary for PCR such as ProFi Taq DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run, by simply adding the template DNA and primers, a high-performance PCR product can be obtained.

※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

$361.00

Cat. No.

K-2632

Product Overview

Features and Benefits

High Efficiency, High Sensitivity PCR Amplification
Guaranteed accurate PCR products with the excellent sensitivity and the best amplification efficiency
Long Range PCR
Effective amplification of large genomic DNA fragments up to 20 kb of human DNA
Convenience
Simplified procedure only requiring the addition of templates and primers with vaccum-dried components
Stability
Enhanced stability allowing maintaince of stable enzymatic activities after long-term storage by including a stabilizer and dried in the PCR reaction mixture
Reproducibility
Minimized deviation between lots through a mass production under ISO 9001 quality system, allowing reproducible results even for replicates tested repeatedly under same conditions and variables

Application

• Primer extension

• long-range amplification from genomic DNA

• High amplification efficiency

• Excellent performance on difficult templates

• Amplification of low-copy targets

• High yield and high sensitivity PCR

Experimental data

Figure. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human Insulin receptor gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human genomic DNA
Lane 2 : 1 ng of human genomic DNA
Lane 3 : 100 pg of human genomic DNA
Lane 4 : 10 pg of human genomic DNA

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	Yes
3'- A overhang	Yes
PCR product size	~ 30 kb

▶ Experimental data

Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower^® RocketScript ™ Cycle RT PreMix.(Bioneer, Cat. No K-2201) wase used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human GAPDH gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA

Figure 2. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 2 kb fragment (human tumor protein p53 gene)
Lane 2 : 3 kb fragment (human tumor protein p53 gene)
Lane 3 : 4.5kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene

Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 11 kb fragment
Lane 2 : 13.5 kb fragment
Lane 3 : 17.6 kb fragment
Lane 4 : 21.4 kb fragment

Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 15 kb fragment
Lane 2 : 20 kb fragment
Lane 3 : 25 kb fragment
Lane 4 : 30 kb fragment

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description	Option			price
K-2631	AccuPower^® ProFi Taq PCR PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes	20 μl reaction	$K-2631
K-2633				50 μl reaction	$K-2633
K-2632			0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes	20 μl reaction	$K-2632
K-2634				50 μl reaction	$K-2634
K-2635		MasterMix type	1 ml of 2X Master mix solution		$K-2635