AccuPower® RocketScript™ RT-PCR PreMix is a one-step RT-PCR product that uses Thermostable RTase (RocketScript™ Reverse Transcriptase) and vacuum-dried ProFi Taq DNA polymerase, dNTP, reaction buffer, etc. By using the RocketScript™ Reverse Transcriptase, RT reactions can be performed at a higher temperature for obtaining template RNA with strongly bounded secondary structure. Not only it has high elongation and sensitivity, but also contains all the components necessary for cDNA synthesis and electrophoresis, including the tracking dyes, to perform RT-PCR reaction directly just by adding the RNA, primer, and D.W.
※This product is shipped in dry ice.
Synthesis of full-length size of 5.2 kb (target: human TFRC) target through one-step RT-PCR using human total RNA 100 ng
Synthesis using only 10 pg of human total RNA pg (target: ß-Actin) extracted from cells and tissues through one-step RT-PCR
Excellent thermal resistance of RocketScript ™ RTase active even at 70℃, making it suitable for cDNA synthesis of secondary RNA structure with comprehensive selection of compatible temperature ranging from 42 to 70 ℃ depending on the required experimental condition and effective cDNA synthesis
Note: Schematic representation of the 5'UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript™ shows full activity at 70℃ allowing it to synthesize the complete gene sequence where M-MLV and other reverse transcriptase's fail.
Mass produced in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results
All necessary components for cDNA synthesis such as thermostable reverse transcriptase, RNase inhibitor, etc., included to perform the reaction immediately just by adding the template RNA to be amplified, primer, and D.W.
Tracking dye and sedimentation agents also included in the products for electrophoresis, making the loading buffer unnecessary to be added
• First-strand synthesis of cDNA from RNA molecules (RT)
• RT-PCR
• cDNA library construction
• Gene expression analysis
Figure. Performance comparison between AccuPower® RocketScript ™ RT-PCR PreMix and competitor RT-PCR kits
Complex secondary structure RNA species amplification by RocketScript ™ is outstanding compared to the leading competitor's reverse transcriptase. RT reactions were performed according to each manufacturer's recommendations.
Lanes 1-4 are 10 ng, 1 ng, 0.1 ng and 0.01 ng of total RNA from HeLa cells, respectively
