AccuPower® RocketScript™ RT-PCR Master Mix, RNase H Minus (2X, 1ml)

AccuPower^® RocketScript™ RT-PCR Master Mix, RNase H Minus is the Master Mix type of AccuPower^® RocketScript™ RT-PCR PreMix, RNase H Minus products.

*Master Mix product is shipped in dry ice

$311.00

Cat. No.

K-2235

Product Overview

Reaction principle

AccuPower^® RocketScript™ RT-PCR PreMix, RNase H Minus is a one step RT-PCR product containing RocketScript™ RTase, RNase H Minus, and ProFi Taq DNA polymerase with excellent processivity and fidelity, which can react at 70℃. By just using a single tube, both cDNA synthesis and two-step PCR protocol can be done to accurately produce the desired base sequences.

Features and Benefits

Thermostable Activity
Excellent thermal stability of RocketScript™ RTase, RNase H Minus retaining activity at 70℃, making it suitable for cDNA synthesis of secondary structure RNA.
RNase H- Activity
Inhibiton of RNase H activity, capable of degrading RNA / DNA hybrid generated during cDNA synthesis in RocketScript™ RTase RNase H Minus for an effective long target RNA
Sensitivity & Fidelity
Highly sensitive and accurate Profi Taq DNA polymerase capable of amplifying small amounts of large RNA.
User-friendly
Simple amplification of long-size RNA difficult to be done with conventional one-step RT-PCR methods

Application

• Standard RT-PCR

• Long kb RT-PCR

• Virus detection

• Gene-expression analysis

Experimental data

Figure. Performance of the AccuPower^® RocketScript ™ RT-PCR PreMix, RNase H Minus

AccuPower^® RocketScript ™ RT-PCR PreMix, RNase H minus was used to amplify cDNAs which size are between 2.5 kb to 12.5 kb. DNA sizes are indicated above the picture. Reactions were performed with 100 ng total RNA from HeLa cells.

M1: Lambda/Hind III marker (Cat. No. D-1050, Bioneer)
M2: 1kb DNA Ladder (Cat. No. D-1040, Bioneer)

Technical/Specs

▶ Specifications

Enzyme	RocketScript ™ RTase, RNase H Minus ProFi Taq DNA polymerase
DNase activity	No
RNase activity	No
RNase H activity	No
Fragment size	Up to 12.5 kb

▶ Experimental data

Figure 1. Comparison of thermal stability between RocketScript ™ RT-PCR PreMix, RNase H Minus and other suppliers’ RT-PCR kits

RocketScript ™ RT-PCR PreMix, RNase H Minus, as well as other competitors’ RT-PCR kits, were used to synthesize cDNA at a temperature range of 40–70°C, It is shown that RocketScript ™ RT-PCR PreMix, RNase H Minus provides increased specificity and higher yields of cDNA than other suppliers’ kits.

M: 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Lane 1~4: 10 ng, 1 ng, 100 pg, 10 pg Human total RNA from HeLa cells, respectively.

Figure 2. Comparison of Sensitivity between RocketScript ™ RT-PCR PreMix, RNase H Minus from Bioneer and other suppliers’ RT-PCR kits

M: 100 bp DNA Ladder (Cat. no, D-1030, Bioneer)
Lane 1-4: 10 ng, 1 ng, and 100 pg, 10 pg of total RNA from HeLa cells, respectively.

Figure 3. Sensitivity comparison of the various targets

Reverse transcription conditions: Conventional 30 min incubation at 50℃, deactivation at 95℃ for 5 min

M: 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Lane 1-2: 100 ng and 10 ng of total RNA from HeLa cells, respectively.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

Cat. No.	Product Description	Option			Price
K-2231	AccuPower^® RocketScript™ RT-PCR PreMix & Master Mix ,RNase H Minus	PreMix type	0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	20 µl/rxn	$K-2231
K-2233			0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	50 µl/rxn	$K-2233
K-2232			0.2 ml thin-wall 8-tube strips with attached cap / 480 tubes	20 µl/rxn	$K-2232
K-2234				50 µl/rxn	$K-2234
K-2235		Master Mix type	1 ml of 2X Mastermix solution		$K-2235