AccuPower® RocketPlex RT-PCR Master Mix (2X, 1ml)

AccuPower^® RocketPlex RT-PCR Master Mix is the Master Mix type of AccuPower^® RocketPlex RT-PCR Premix products.

※ Master Mix product is shipped in dry ice

$291.00

Cat. No.

K-2217

Product Overview

Features and Benefits

Superior Multiplex Performance
Simultaneous cDNA synthesis and amplification up to 10 target genes just by adding their primers in a single tube

Single and Multiplex RT-PCR
Specificity
HotStart Taq DNA Polymerase for increasing the specificity of the PCR reaction.
User-friendly
Amplification of various targets easily in a single tube with one reaction which can be used by anyone

Application

• Multiplex RT-PCR

• Low copy detection

• Gene-expression analysis

Experimental data

Figure. Single RT-PCR and Multiplex RT-PCR using AccuPower^® RocketPlex RT-PCR PreMix
M; 25/100 bp Mixed DNA Ladder (Cat. No. D-1020,Bioneer)

Technical/Specs

▶ Specifications

5' to 3' exonuclease	Yes
3' to 5' exonuclease	No
3' – A overhang	Yes
Fragment Size	Up to 1 kb

▶ Experimental data

Figure 1. Comparison of amplification quality between AccuPower^® RocketPlex PCR PreMix and other suppliers' multiplex PCR kit.
10-plex primers were added into AccuPower^® RocketPlex RT-PCR PreMix and other suppliers' RT-PCR kit. A series of human genomic RNA diluents were tested (Lane 1; Human genomic RNA 100 ng, Lane 2; Human genomic RNA 10 ng, Lane 3; Human genomic RNA 1 ng).
Reverse transcription conditions: conventional 1 hr incubation at 42°C, 50°C, 55°C, 60°C and 65°C deactivation at 95°C for 5 min
All data were obtained using MyGenie™ 96 machine (Applied Bioneer co.)
AccuPower^® RocketPlex RT-PCR PreMix is able to perform reverse transcription reactions throughout a wide range of temperatures from 42°C to 65°C.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description	Option			Price
K-2211	AccuPower^® RocketPlex RT-PCR PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	20 μl reaction	$K-2211
K-2213			0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes	50 μl reaction	$K-2213
K-2212			0.2 ml thin-wall 8-tube strips with attached cap / 480 tubes	20 μl reaction	$K-2212
K-2214				50 μl reaction	$K-2214
K-2217		MasterMix type	1 ml of 2X Master mix solution		$K-2217