AccuPower® Multiplex PCR Master Mix (2X, 1ml)

▶Specifications

▶ Experimental data

Figure 1. Single PCR and multiplex PCR using AccuPower^® Multiplex PCR PreMix

Each lane from left to right indicates the single and multiplex PCR product using AccuPower^® Multiplex PCR PreMix.
a) 10 plex multiplex PCR, b) 20 plex multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)

Figure 2. Comparison of amplification quality between AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

6-plex primers were added into AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of Human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl₂).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 60°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)

Figure 3. Comparison of amplification quality using Lapchip^® between AccuPower^® Multiplex PCR PreMix and other supplier's master mix.

a) Virtual gel image. Gel image illustrates data reproducibility of the Lapchip^® 90 system.
b) Overlay of expression level using Bioneer's multiplex PCR PreMix and other company's multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower^® Multiplex PCR PreMix and other supplier's master mix.

Figure 4. Comparison of amplification quality between AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

20-plex primers were added into AccuPower^® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (100 ng ~ 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.)
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl₂)
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 57°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer) Trademark: 'AccuPower^®' is a registered trademark of Bioneer Corporation.

◈ Manual

• Manual_AccuPower^® Multiplex PCR PreMix

• Manual_AccuPower^® Multiplex PCR Master Mix

◈ MSDS

• MSDS_AccuPower^® Multiplex PCR PreMix

◈ Brochure

• All about PCR

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate