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RocketScript™ Reverse Transcriptase, RNase H Minus (50,000 units)
This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.
※This product is shipped in dry ice.
Features and Benefits
- Long cDNA Synthesis
Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H
- Thermostability
Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products
- Sensitivity
Excellent amplification sensitivity even with a trace amount of 1 pg RNA
- Reproducibility
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
Application
- Gene synthesis
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First-strand synthesis of cDNA from RNA molecules (reverse transcription)
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Random priming reactions
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Library construction
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Probe labeling
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mRNA 5’-end mapping by primer extension analysis
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Real-time PCR
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RT-PCR
Experimental data
Figure. Amplification results of RocketScript ™ Reverse Transcriptase, RNase H Minus. (A) Sensitivity (B) Thermostability.
Lane M; 100 bp DNA Ladder(Bioneer, Cat. No. D-1030), Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell, Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell, Lane 5; 10 pg human total RNA from HeLa cell