This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.
※This product is shipped in dry ice.
Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H
Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products
Excellent amplification sensitivity even with a trace amount of 1 pg RNA
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
First-strand synthesis of cDNA from RNA molecules (reverse transcription)
Random priming reactions
Library construction
Probe labeling
mRNA 5’-end mapping by primer extension analysis
Real-time PCR
RT-PCR
Figure. Amplification results of RocketScript ™ Reverse Transcriptase, RNase H Minus. (A) Sensitivity (B) Thermostability.
Lane M; 100 bp DNA Ladder(Bioneer, Cat. No. D-1030), Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell, Lane 3; 1 ng human total RNA from HeLa cell
Lane 4; 100 pg human total RNA from HeLa cell, Lane 5; 10 pg human total RNA from HeLa cell
