HotStart DNA polymerase with dNTPs (1,000 units)

HotStart DNA polymerase inhibits PCR by using pyrophosphate (PPi) to trap Mg2+ ions essential for PCR, then breaking the PPi down by using heat resistant enzyme pyrophosphatase (PPase) as the temperature increases while processing the PCR reaction 

※This product is shipped in dry ice.

$385.00
Cat. No.
E-3151

Principles of HotStart DNA Polymerase

HotStart DNA Polymerase is a new concept of DNA polymerase that utilizes chemical interactions between pyrophosphate (PPi) and pyrophosphatase (PPase).
While divalent cation magnesium (Mg2+) plays an important role in PCR, the pyrophosphate binds with Mg2+ to form a complex that inhibits the PCR.
 However, the Mg-PPi complex will decompose into Mg2+ and 2Pi by the thermostable pyrophosphatase (PPase) during the PCR reaction, allowing increased reactivity with the by-product of the digested PPi, 2Pi. 

 

Features and Benefits

  • Sensitivity & specificity

    Reduced generation of non-specific responses and primer-dimer formation during the mixing of PCR components by inhibiting DNA polymerase activity at the room temperature

  • Efficiency 

    Improved yield of target DNA through its high sensitivity and specificity

  • Reproducibility

    Reproducible results with uniform quality products for each batch under the ISO 9001 quality system

Application

  • HotStart PCR, PCR with complex genomic templates/low copy templates/cDNA
  • Multiplex PCR
  • Primer extension
  • SNP typing
  • Real-time PCR using dsDNA Binding dye
  • Multiple primer pairs and amplification of low copy template DNA

Experimental data



Figure. Multiplex PCR comparison of genomic DNA using 6 sets of primers.

Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)

Lane 1 : 750 bp fragment

Lane 2 : 590 bp fragment

Lane 3 : 450 bp fragment

Lane 4 : 360 bp fragment

Lane 5 : 260 bp fragment

Lane 6 : 150 bp fragment

Lane 7 : Multiplex PCR with primers used for Lane 1 – 6

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