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RocketScript™ Reverse Transcriptase (50,000 units)
RocketScriptTM Reverse Transcriptase is an independently developed thermostable reverse transcriptase derived from M-MLV. Its resistnace to heat (42 ~ 70℃) allows effective synthesis of complex RNA structures with full-length cDNA.
※This product is shipped in dry ice.
Features and Benefits
- Thermostablity
Performance at comprehensive temperature conditions from 42 to 70 ℃ for cDNA synthesis with Rocketscript ™ series overcoming the limitation of traditional 42°C RT reactions
- User-friendly
Simplified procedure by including all components necessary for cDNA synthesis such as Thermostable Reverse Transcriptase, RNase inhibitor, etc.
- Reproducibility
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
Application
- Gene synthesis
- First-strand synthesis of cDNA from RNA molecules (reverse transcription)
- RT-PCR
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5'-end mapping by primer extension analysis
- Real time PCR
Experimental data
Figure. Complex RNA amplification.
Complex RNA amplification results of RocketScript™ Reverse Transcriptase compared with that of conventional RTase.
Reverse transcription condition: conventional 42°C/50°C/60°C 1 hr, deactivation 95°C 5 min, This product shows thermal stability.
Lane A; M-MLV Reverse Transcriptase
Lane B; RocketScript™ Reverse Transcriptase
Lane 1; 100 ng human total RNA from HeLa cell
Lane 2; 10 ng human total RNA from HeLa cell