T7 RNA Polymerase (5,000 unit)

T7 RNA polymerase a product purified after being synthesized by expressing the gene for bacteriophage T7 RNA polymerase in Escherichia coli.
This allows to specifically bind onto the T7 phage promoter site and catalyze the RNA synthesis from the cloned DNA sequence located at the bottom of the T7 promoter site.

$88.00

Cat. No.

E-3043

Product Overview

Features and Benefits

High Specificity
Has high specificity to T7 phage promoter sequence allowing synthesis of desired RNA transcriptome.
Versatility
Conveniently synthesize the mRNA and sgRNA for such as for vaccine production and CRISPR-Cas9 researches.
Reproducibility
Manufactured under ISO9001 Quality management systems to produce each batch with unformed quality, allowing reproducible results.

Application

Synthesis of highly radiolabeled RNA probes
Synthesis of precursors of siRNA
Synthesis of precursors for RNA splicing reactions
Synthesis of mRNA for in vitro translation
Synthesis of sgRNA for gene targeting
RNA structure, processing and catalysis studies
Expression control via antisense RNA
Vaccines, CRISPR-Cas9

Technical/Specs

▶ Specifications

DNase activity	No
RNase activity	No
Protease activity	No

▶ Components

• 5X Reaction buffer with MgCl₂: 200 mM Tris-HCl, 30 mM MgCl₂, 10 mM spermidine, pH 8.0

• RNase Inhibitor: 100 ng/µl

• 100 mM DTT

▶ Concentration

5,000 units (50 U/μl)

▶ Storage conditions

50% (v/v) glycerol containing 20 mM sodium phosphate, 100 mM KCl, 0.5 mM EDTA, 1 mM DTT, Stabilizers, pH 7.7

▶ Storage temperature

-20°C

▶ Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 60 minutes at 37℃

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option		Price
E-3043	T7 RNA Polymerase	5,000 U	10X Reaction Buffer with MgCl₂	$E-3043
E-3044	T7 RNA Polymerase	25,000 U	10X Reaction Buffer with MgCl₂	$E-3044