HotStart Taq DNA Polymerase, 10X Reaction Buffer with 15 mM MgCl2, 10 mM dNTP (250 units)

HotStart Taq DNA Polymerase prevents non-specific amplification caused by mispriming or primer-dimer formation, which may occur during the mixing of PCR reactants at the room temperature.

※This product is shipped in dry ice.

$110.00

Cat. No.

E-2017

Product Overview

Features and Benefits

Sensitivity
High sensitivity capable of undergoing PCR with a small amount of template by reducing unnecessary reactions
Specificity
Reduced generation of non-specific priming by inhibiting DNA polymerase activity at room temperature while mixing the PCR reactants
Reproducibility
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system

Application

Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
HotStart PCR
Mutiplex PCR
Automated PCR

Experimental data

Figure. Specificity comparison between Hotstart.Taq DNA polymerase and other companies products.

M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 139 bp         Lane 5; 1,082 bp
Lane 2; 211 bp         Lane 6; 1,296 bp
Lane 3; 447 bp Lane 7; 1,561 bp
Lane 4; 618 bp         Lane 8; Multiplex PCR (447 bp, 618 bp)

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 10 kb

▶ Components

• 10X reaction buffer : Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0

• Dilution buffer : 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50% Glycerol, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration

250 U/50 µl

▶ Storage conditions

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50% Glycerol, pH 8.0

▶ Storage temperature

-20°C

▶ Unit definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

▶ Experimental data

Figure 1. HotStart Taq DNA polymerase의 specificity test. Single and multiple PCR results in human genomic DNA p53 gene amplification

M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 139 bp         Lane 5; 1,082 bp
Lane 2; 211 bp         Lane 6; 1,296 bp
Lane 3; 447 bp Lane 7; 1,561 bp
Lane 4; 618 bp         Lane 8; Multiplex PCR (139 bp, 447 bp, 618 bp)

Literature/Support

◈ Manual

• Manual_HotStart Taq DNA Polymerase

◈ MSDS

• MSDS_10 mM dNTPs

• MSDS_10X Reaction buffer for HotStart Taq DNA polymerase

• MSDS_Dilution buffer for HotStart Taq DNA polymerase

• MSDS_HotStart Taq DNA Polymerase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option			Price
E-2017	HotStart Taq DNA Polymerase	250 U	10X Reaction Buffer with 15 mM MgCl₂	10 mM dNTP	$E-2017
E-2017-3		250 U	10X Reaction Buffer with 15 mM MgCl₂		$E-2017-3
E-2017-2		500 U	10X Reaction Buffer with 15 mM MgCl₂	10 mM dNTP	$E-2017-2
E-2017-1		1,000 U	10X Reaction Buffer with 15 mM MgCl₂	10 mM dNTP	$E-2017-1
E-2017-4		1,000 U	10X Reaction Buffer with 15 mM MgCl₂		$E-2017-4