Taq DNA Polymerase, 10X Reaction Buffer with MgCl2 (2,000 units)

Taq DNA polymerase is a thermostable polymerase derived from the gene of Thermus aquaticus YT1 expressed and purified from Escherichia coli .

※This product is shipped in dry ice.

$249.00

Cat. No.

E-2013-2

Product Overview

Features and Benefits

Efficiency & Sensitivity

High efficiency and sensitivity to the templates
Optimized buffer delivery
Stable PCR reaction by providing buffers optimized for the enzyme
Reproducibility
Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system

Application

Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

Experimental data

Figure 1. TaqSensitivity test of DNA Polymerase.
Validation of enzymatic activities by using 1 unit of Taq DNA polymerase on: (A) human genomic DNA (B) Lambda DNA

M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 100 ng template DNA
Lane 2; 10 ng template DNA
Lane 3; 1 ng template DNA
Lane 4; 100 pg template DNA
Lane 5; 10 pg template DNA
Lane 6; 1 pg template DNA
Lane 7; 100 fg template DNA

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 10 kb

▶ Components

• 10X reaction buffer with (or without) MgCl₂: Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0

• Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration

500 units (5 U/μl)

▶ Storage conditions

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0

▶ Storage temperature

-20°C

▶ Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.

▶ Experimental data

Figure 1. Serial dillution test of Taq polymerase with dilution by 1/5, 1/10, 15, 1/20 to use them on either human genomic DNA (A) or Lambda DNA (B) as a template for PCR

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 U of Taq DNA polymerase
Lane 2; 1 U of Taq DNA polymerase
Lane 3; 0.5 U of Taq DNA polymerase
Lane 4; 0.33 U of Taq DNA polymerase
Lane 5; 0.25 U of Taq DNA polymerase

Figure 2. Long kb DNA amplification test by Taq DNA Polymerase.
Amplification done from 5 kb to 8 kb using 20 pg of Lambda DNA as a template

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 kb PCR product
Lane 2; 6 kb PCR product
Lane 3; 7 kb PCR product
Lane 4; 8 kb PCR product

Literature/Support

◈ Manual

• Manual_Taq DNA Polymerase

• Manual_10X Reaction buffer for Taq DNA polymerase

◈ MSDS

• MSDS_10 mM dNTPs

• MSDS_20 mM MgCl₂

• MSDS_10X Reaction buffer without MgCl2 for Taq DNA polymerase

• MSDS_10X Reaction buffer for Taq DNA polymerase

• MSDS_Dilution buffer for Taq DNA polymerase

• MSDS_Taq DNA polymerase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option			Price
E-2011	Taq DNA Polymerase	500 U	10X Reaction Buffer with MgCl₂	10 mM dNTP	$E-2011
E-2011-2			10X Reaction Buffer with MgCl₂		$E-2011-2
E-2011-1			10X Reaction Buffer, without 20 mM MgCl₂	10 mM dNTP	$E-2011-1
E-2011-3			10X Reaction Buffer, without 20 mM MgCl₂		$E-2011-3
E-2013		2,000 U	10X Reaction Buffer with MgCl₂	10 mM dNTP	$E-2013
E-2013-2			10X Reaction Buffer with MgCl₂		$E-2013-2
E-2013-1			10X Reaction Buffer, without 20 mM MgCl₂	10 mM dNTP	$E-2013-1
E-2013-3			10X Reaction Buffer, without 20 mM MgCl₂		$E-2013-3