Overview
Recently, there have been many researches applying magnetic beads in the field of biology: from cellular experiments and DNA purification to disease monitoring and even disease treatment. The surface of magnetic beads are coated with functional groups that allows binding of DNA, RNA, proteins, and specific cells. Afterwards, the beads are moved or attached to a desired position through external magnetic fields to perform purification.
Features and Benefits
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Miniscule size
1 - 5 um sizes of magnetic beads
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Uniform disperse size distribution
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Sphere form of silica coated
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Excellent DNA/RNA purification efficiency
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Rapid purification
Applications
• Magnetic DNA/RNA Purification
• Magnetic Protein Separation
• Magnetic Antibody Separation
• Magnetic Cell Isolation
▶ Efficient Magnetic Beads Purification Method for DNA Separation
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Uses uniformed superparamagnetic magnetic beads and DNA/RNA affinity.
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Mix magnetic beads and DNA/RNA with buffer containing salts.
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Bind DNA/RNA to Magnetic Beads.
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Seperate DNA/RNA with high efficiency within a few minutes by external magnetic field.
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Does not require centrifugation, vacuum, filtration, making it suitable to be used with automated procedures.
▶ Application to DNA/RNA purification
After lysing the sample, add the magnetic beads to combine them with the degraded DNA. Afterwards, fix the beads by using external magnetic field and wash the solution. Finally, elute the DNA or RNA from the magnetic beads. This method provides faster DNA purification than traditional ones.
Figure 1. Separation of DNA using silica-coated magnetic beads
▶ FE-SEM Images of Magnetic Beads
Figure 2. FE-SEM image of Magnetic Beads for DNA/RNA Purification.
Electron micrographs of the silica coated magnetic particles show that the beads are unformly in size of 1-2 μm.
Figure 3. Extended FE-SEM image of Magnetic Beads.
Electron microscope image shows the spherical magnetic beads completely coated with silica.
▶ Size Distribution and Zeta Potential Diagram of Magnetic Beads
Figure 4. Particle Size Distribution and Zeta Potential of Silica coated Magnetic Beads.
The average particle size of the magnetic beads is 1.56um, and the zeta potential of the magnetic beads is -39.8 mV due to the hydroxyl on the silica surface.
◈ Brochure
• AccuBead Silica Coated Magnetic Beads
◈ MSDS
• MSDS of AccuBead Silica Coated Magnetic Beads
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 – certificate
◈ Technical Support and Ordering
For more information on our products, please contact us by email.
nano-support@bioneer.com
▶ Ordering Information
Silica Coated Magnetic Beads is a research reagent for the separation of biomaterials (DNA, RNA, Protein, Cell). Package sizes of Magnetic Beads are 0.5g, 1g and 10g.
* It is shipped in ice packing.
** Please inquiry for bulk orders.
▶ Technical Support and Ordering
For more information on our products, please contact us by email.
nano-support@bioneer.com
