Specifications
| Instrument |
AllInOneCycler™ Thermal Block or other Thermal Block |
| Specimen |
Feces small and large intestines septicemia urine other svstemic organs |
| Time to result |
1h 45min (Post-extraction) |
| Tests per Kit |
48 |
Performances
Figure 1. Electrophoresis of PCR product by AccuPower® Salmonella spp. 4-Plex PCR kit.
Using AccuPower® Salmonella spp. 4-Plex PCR Kit, Total of 4 ng template DNA [ATCC 13076(S. Enteritidis), ATCC 7001 (S. Choleraesuis), ATCC 14028 (S. Typhimurium) 2 ng each)] was PCR reacted to 95°C 5 min., 95°C 30 sec. 65°C 1 min 30 sec (30 cycles), 72°C 5 min. 3 ul of amplified PCR product was ran on 2% TBE Agarose gel. Lane M: Size Marker(MSE-1115 Size Marker)
Lane P: Positive control
Lane N: Non-template control
Figure 2. Specificity test for 20 species of wild samplesUsing
AccuPower®Salmonella spp. 4-Plex PCR Kit and other products, 4 ng of various intestinal bacterial genomic DNA was PCR reacted to 95℃ 5 min, 95℃ 30 sec 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min. 3 ul of amplified PCR product was ran on 2% TBE Agarose gel.Lane 1, 5, 9, 10, 14, 16 & 17: S. Typhimurium (307bp) Lane 2, 3, 7, 15 & 19: S. Enteritidis (627bp)
Lane 8, 12 & 18: S. Cholerasuis (409bp)
Lane 4: S. Rissen Lane 6: S. Montevideo Lane 11: S. Schwarzengrund
Lane 13: S. Gallinarum Lane 20: S. Derby
Lane M: 100bp DNA Ladder (D-1030)
Lane N: No Template Control
Figure 3. Sensitivity experiment for AccuPower® Salmonella spp. 4-Plex PCR Kit
A total of 6 ng of Genomic DNA (Cyclic SE, ST & SC DNA of 2 ng) was PCR reacted to 95℃ 5 min, 95℃ 30 sec 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min. 3 ul of amplified PCR product was ran on 2% TBE Agarose gel. AccuPower® Salmonella spp. 4-Plex PCR Kit can detect up to 1 × 104 CFU / ml.
Lane 1: 1 x 108 CFU/ml
Lane 2: 1 x 107 CFU/ml
Lane 3: 1 x 106 CFU/ml
Lane 4: 1 x 105 CFU/ml
Lane 5: 1 x 104 CFU/ml
Lane 6: 1 x 103 CFU/ml
Lane 7: 1 x 102 CFU/ml
Lane M: 100 bp DNA Ladder (D-1030)
Lane N: Negative control
Figure 4. Specificity test for 15 species of intestinal bacteria
A total of 4 ng of Genomic DNA was PCR reacted to [I: 95℃ 5 min., 95℃ 30 sec. 65℃ 1 min 30 sec(30 cycles), 72℃ 5 min, II: 95℃ 5 min., 94℃ 1 min 54℃ 30 sec 72℃ 30 sec(35 cycles), 72℃ 10 min, III: 95℃ 12 min., 94℃ 30 sec 63℃ 30 sec 72℃ 30 sec(35 cycles), 72℃ 10 min]. 3 ul of amplified PCR product was ran on 2% TBE Agarose gel. Lane 1: Acinetobacter lwoffii genomic DNA
Lane 2: Enterococcus fecalis genomic DNA
Lane 3: Maltophilia genomic DNA
Lane 4: Pseudomonas putida genomic DNA
Lane 5: Proteus mirabilis genomic DNA
Lane 6: Citrobacter freundii genomic DNA
Lane 7: Klebsiella pneumonia sub. genomic DNA
Lane 8: Enterobacter cloacae genomic DNA
Lane 9: Acinetobacter haemolyticus genomic DNA
Lane 10: Comamonas testosterone genomic DNA
Lane 11: Clostridium perfrigens alpha genomic DNA
Lane 12: Burkholderia cepacia genomic DNA
Lane 13: Ochrobactrum anthropi genomic DNA
Lane 14: Serratia marcescens genomic DNA
Lane 15: Acinetobacter junii genomic DNA
Lane M: 100 bp DNA Ladder (D-1030)
Lane N : Negative control
