Bacterial respiratory disease in pigs takes place all over the world. It usually becomes worse due to stress from poor ventilation and temperature change, as well as secondary infection after viral infection. It causes economic damages arising from death and reduced productivity. Typical pathogens include
Actinobacillus pleuropneumoniae (App), Actinobacillus suis (A. suis), Haemophilus parasuis (H. Parasuis) and Streptococcus suis (S. Suis). Actinobacillus pleuropneumoniae (App), a pathogen for pleuropneumonia, causes sudden death in adult and immature pigs.
Actinobacillus suis (A. suis) normally stays in porcine tonsil, nasal cavity, or vagina, and it causes acute sepsis, pneumonia, arthritis, abortion and swine erysipelas in poor immune status or worse management condition. Haemophilus parasuis (H. parasuis), the Causal Body of Glasser's Disease, causes respiratory pneumonia and systemic polyserositis. A growing pig usually shows symptoms such as sneezing, anorexia, high fever, a weight loss, joint swelling, conjunctivitis, etc. During chronic progression, mortality rate as high as 10~50% has been reported. Streptococcus suis (S. suis) infection causes porcine respiratory disease most frequently among Streptococcus species and also develops into pneumonia, meningitis, arthritis, chronic bronchitis, etc.
| |
AccuPower® Porcine Respiratory
4-Plex PCR Kit |
Standard Diagnostic Method for Animal Disease (QIA) |
A company (France) |
B company (Chile) |
| App |
+ (Multiplex) |
+(Singleplex) |
+(Singleplex) |
+(Singleplex) |
| A. suis |
- (Not detected) |
- (Not detected) |
- (Not detected) |
| H. parasuis |
+(Singleplex) |
- (Not detected) |
+(Singleplex) |
| S. suis |
+(Singleplex) |
- (Not detected) |
- (Not detected) |
| Notes |
LoD: 4x104 cfu/ml
Using a preMix dried kit |
Singleplex PCR
Using a solution mix kit |
* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea
Features
Developed and patented in cooperation with QIA
> Fast detection
Less than 2 hour simultaneous detection of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis through a 2 step PCR reaction in a single tube.
> Optimized sensitivity and specificity
Confirmed on the 143 in-field strains owned by QIA. By applying patented enzyme-mediated HotStart technology, achieved a sensitivity of 4x104 CFU/ml with possible exclusion of false potisive.
> Excellent reproducibility
Use of dried-type PCR PreMix only requires simple addition of template DNA and DW, which provides highly reproducible results.
> Prevention of amplicon-induced cross-contamination by 8-MOP addition
> High Stability
1-year warranty under -20 °C.
> Quality Control
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
