Riemerella anatipestifer, E. coli and Salmonella spp. cause a systemic disease in chickens, ducks and other poultry that reduces weight and hatchability in them, which finally results in an enormous damage to farmers. E. coli is mostly related to repiratory diseasee in poultry, which may develop into sepsis, granuloma, air sacculitis, salpingitis and arthritis that cause tremendous economic loss. Riemerella anatipestifer is a nonmotile, gram-negative bacillus, and 21 serotypes have been identified to date —in South Korea, 1, 4, 7 and 16 serotype. It causes acute and chronic septic infectious diseases characterized by fibrous pericarditis, perihepatitis, air sacculitis and meningitis, which result in high mortality and weight loss in poultry. Salmonella causes S. Pullorum, S. Gallinarum and avian paratyphoid infection in poultry that are followed by a fall in egg production, fertility or hatchability, or even a death.
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AccuPower® Bacterial Poultry
3-Plex PCR Kit |
Standard Diagnostic Method for Animal Disease (QIA*) |
I company (korea) |
B company (Chile) |
G company (Germany) |
| E. coli |
+ (Multiplexing) |
- (Not detected) |
- (Not detected) |
- (Not detected) |
+( Singleplex) |
| R. anatipestifer |
+( Singleplex) |
- (Not detected) |
- (Not detected) |
- (Not detected) |
| Salmonella spp. |
+( Singleplex) |
+( Singleplex) |
+( Singleplex) |
+( Singleplex) |
| notes |
LoD: 104 cfu/ml
Using a PreMix dried kit |
Singleplex PCR
Using a solution mix kit |
* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea
Features
Developed and patented in cooperation with QIA
> Fast detection
Less than 2 hour simultaneous detection of Riemerella anatipestifer, E. coli and Salmonella spp. through a 2 step PCR reaction in a single tube.
> Optimized sensitivity and specificity
Confirmed on the 60 in-field strains owned by QIA. By applying patented enzyme-mediated HotStart technology, achieved a sensitivity of 1x104 CFU/ml with possible exclusion of false potisive.
> Excellent reproducibility
Use of dried-type PCR PreMix only requires simple addition of template DNA and DW, which provides highly reproducible results.
> Prevention of amplicon-induced cross-contamination by 8-MOP addition
> High Stability
1-year warranty under -20 °C.
> Quality Control
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
