AccuPower® ETEC-Pili 5-Plex PCR Kit
AccuPower® ETEC-Pili 5-Plex PCR Kit provides one-step detection of 5 types of pilis produced by ETEC E. coli in animals: F4 (K88), F5 (K99), F6 (987P), F18 and F41. This featured kit is comprised of all the essential requirements for PCR reactions including primer, salts, dNTPs, and stabilizers in one tube to the provivded ready-to-use Premix format product. Users only need to add template DNA and D.W to perform the test.
5-Plex PCR Kit
|Animal Disease Standard
Diagnostic Method (QIA)
|F4(K88)||+ (Detected)||+ (Detected)||Not Identifed same (similar) kit|
|Notes||PreMix dried kit,
Detection limit of 104 cfu/ml
|Master solution Mix|
FeaturesDeveloped cooperatively with QIA (Quarantine and Inspection Agency), Application of patent completed (Patented).
> Fast response
Less than 2-hr detection of 5 pili-type pathogenic E. coli [F4 (K88), F5 (K99), F6 (987P), F18 & F41] through one 2-step PCR reaction in a tube.
Confirmed on the 171 in-field strains owned by QIA. Application of patented Enzyme-mediated HotStart technology: 1x104 CFU/ml of optimized sensitivity, possible exclusion of false potisive.
Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result provided.
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.
1-year warranty under -20 °C.