AccuPower® ETEC-Pili 5-Plex PCR Kit

AccuPower® ETEC-Pili 5-Plex PCR Kit is designed to detect 5 types of E. coli Pili gene simultaneously such as F4 (K88), F5 (K99), F6 (987P), F18 & F41.
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Cat. No.
MAP-1115

 
AccuPower® ETEC-Pili 5-Plex PCR Kit provides one-step detection of 5 types of pilis produced by ETEC E. coli in animals: F4 (K88), F5 (K99), F6 (987P), F18 and F41. This featured kit is comprised of all the essential requirements for PCR reactions including primer, salts, dNTPs, and stabilizers in one tube to the provivded ready-to-use Premix format product. Users only need to add template DNA and D.W to perform the test.

  AccuPower® ETEC-Pili
5-Plex PCR Kit
Animal Disease Standard 
Diagnostic Method (QIA)
Other Kits
F4(K88) + (Detected) + (Detected) Not Identifed same (similar) kit
F5(K99)
F6(987P)
F18
F41
Notes PreMix dried kit, 
Detection limit of 104 cfu/ml
Master solution Mix
 
* QIA: Animal, Plant and Fisheries Quarantine and Inspection Agency, Korea

Features

Developed cooperatively with QIA (Quarantine and Inspection Agency), Application of patent completed (Patented).
  

Fast response
 Less than 2-hr detection of 5 pili-type pathogenic E. coli [F4 (K88), F5 (K99), F6 (987P), F18 & F41] through one 2-step PCR reaction in a tube.

The optimized sensitivity and specificity
 Confirmed on the 171 in-field strains owned by QIA. Application of patented Enzyme-mediated HotStart technology: 1x104 CFU/ml of optimized sensitivity, possible exclusion of false potisive.
 
Great reproducibility
Dried-type HotStart PCR PreMix type – Addition of template DNA and DW only required & highly reproducible result provided.
 
 Prevention of amplicon-induced cross-contamination by 8-MOP
Binding of 8-MOP (8-methoxypsoralen) to DNA double-helix structure, followed by UV irradiation.

> High Stability
1-year warranty under -20 °C.

> Quality Control
All AccuPower® products are manufactured under ISO 9001 Quality Control System.
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