rG(Ib)-CE phosphoramidite

• The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is treatment with AMA reagent(ammonia+methylamine gas) for 3 hours at 40°C or by the use of methylamine gas.

• Cleavage and deprotection procedures for RNA synthesis are similar to DNA synthesis, with an additional step to remove the 2'-OH protecting group.

• The 2'-OH group is protected by a tert-butyldimethylsilyl(TBDMS) group to prevent derivatization and degradation during the synthesis cycle.

• Standard RNA phosphoramidites provide excellent coupling efficiency when used together with either ETT or BTT as an activator.

• The purities of manufactured nucleoside phosphoramidites are determined by ³¹P-NMR and RP-HPLC analysis and controlled to be greater than 99%.

• All reagents are conveniently packaged for ABI instruments. Expedite- and Mermade-compatible vials are also available upon request.

Figure 1. HPLC data : ≥ 99%	Figure 2. 31P-NMR data : ≥ 99%