AccuPower® Dual-HotStart™ RT-PCR PreMix (480 T, 50 μl)

AccuPower® Dual-HotStart™ RT-PCR PreMix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction. cDNA can be efficiently synthesized even with having small amounts of template RNA or being in a secondary structure. Thanks to its high sensitivity, accurate RT-PCR results can be obtained from various kinds of samples. Moreover, this product also contains all the components necessary for performing RT-PCR, allowing it to proceed just by adding the template RNA, primers and D.W.

※This product is shipped in dry ice.

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Features and Benefits

  • Sensitivity

    Detection of samples possible even at trace amounts of a target template in RNA with a high concentration that is generally difficult to detect

  • Comprehensive applications

    Excellent reactivity allowing gaining of accurate RT-PCR results even with template RNA samples of blood or soil samples containing various types of PCR inhibitors

  • Specificity

    The world's first Dual Hotstart RT-PCR reaction using Pyro-Hotstart RT reaction and Hotstart PCR optimized to detect soley the target gene accurately.

  • Convienance

    All components necessary for RT-PCR such as Thermostable DNA polymerase, RTase, dNTPs, etc all included, requiring only the addition of template RNA, primers and D.W.

  • Reproducibility

    Mass produced in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results


• Low copy viral/bacterial pathogen load determination in an earlier stage

• Low copy mRNA amplification

• Low copy target RNA quantification

• RNA amplification for microarray and NGS

Experimental data

AccuPower Dual-Hotstart™ RT-PCR PreMix

Figure. Comparison of PCR amplification specificity between AccuPower® Dual-Hotstart ™ RT-PCR PreMix from Bioneer and other suppliers' Hot start PCR master mix.

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