AccuPower® Dual-HotStart™ RT-qPCR PreMix, Exicycler™ 96 8-well strip (96 T, 50 μl)

AccuPower® Dual-HotStart™ RT-qPCR PreMix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction and enhance the sensitivity to effectively undergo reverse transcription of template RNA even if it is present in a miniscule amount. Moreover, this product is compatible with comprehensive types of devices, including Exicycler™ 96 and those from other companies, by providing optimized tube and plates, along with a 2X master mix type..

※This product is shipped in dry ice.

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Features and Benefits

  • Specificity

    The world's first Dual-HotStart ™ RT-qPCR reaction using PyroHotStart RT reaction and HotStart PCR optimized to detect only the target gene precisely.

  • Sensitivity

    Detection of miniscule amounts of the target templates in a high concentration of RNA samples unable to be done with convientional methods with a wide dynamic range of 10 logs up to 10 ~ 1010 copies.

  • Multiplex

    Compatible with many kinds of fluorescent dye (probes) to detect various kinds of target genes.

  • Comprehensive template RNA detection 

    RocketScriptRTase  included for undergoing RT reaction at high temperature and gaining RT-qPCR results from strong secondary template RNA structures

  • Compatibility

    Excellent reactivity towards template RNA allowing  accurate RT-qPCR results from wide choices of samples such as from blood and soils continaing various PCR inhibitors 

  • Convenience

    All reactants necessary for r RocketScript ™ RTase, HotStart Taq DNA polymerase and cDNA synthesis and qPCR included in each tubes to readily start the one-step RT-qPCR by adding template DNA, probe & primer for target gene, and D.W.

  • Stability

    Stabilizer included in the Real-Time PCR reaction mixture, being more stable the solution type products

  • Reproducibility

    Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables


• Gene expression profiling

• Target RNA quantification

• Microbial detection

• Viral/bacterial pathogen load determination

Experimental data


Figure. High sensitivity of AccuPower® Dual-HotStart ™ RT-qPCR PreMix.
Experiment with HIV target. 10 fold serial dilution of  Template RNA (1010 copies ~ 10 copies spiked in Human Total RNA).

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