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AccuPower® Dual-HotStart™ RT-qPCR PreMix, Exicycler™ 96 8-well strip (96 T, 50 μl)
AccuPower® Dual-HotStart™ RT-qPCR PreMix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction and enhance the sensitivity to effectively undergo reverse transcription of template RNA even if it is present in a miniscule amount. Moreover, this product is compatible with comprehensive types of devices, including Exicycler™ 96 and those from other companies, by providing optimized tube and plates, along with a 2X master mix type..
※This product is shipped in dry ice.
Overview
Features and Benefits
- Specificity
The world's first Dual-HotStart ™ RT-qPCR reaction using PyroHotStart RT reaction and HotStart PCR optimized to detect only the target gene precisely.
- Sensitivity
Detection of miniscule amounts of the target templates in a high concentration of RNA samples unable to be done with convientional methods with a wide dynamic range of 10 logs up to 10 ~ 1010 copies.
- Multiplex
Compatible with many kinds of fluorescent dye (probes) to detect various kinds of target genes.
- Comprehensive template RNA detection
RocketScript ™ RTase included for undergoing RT reaction at high temperature and gaining RT-qPCR results from strong secondary template RNA structures
- Compatibility
Excellent reactivity towards template RNA allowing accurate RT-qPCR results from wide choices of samples such as from blood and soils continaing various PCR inhibitors
- Convenience
All reactants necessary for r RocketScript ™ RTase, HotStart Taq DNA polymerase and cDNA synthesis and qPCR included in each tubes to readily start the one-step RT-qPCR by adding template DNA, probe & primer for target gene, and D.W.
- Stability
Stabilizer included in the Real-Time PCR reaction mixture, being more stable the solution type products
- Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables
Application
• Gene expression profiling
• Target RNA quantification
• Microbial detection
• Viral/bacterial pathogen load determination
Experimental data
Figure. High sensitivity of AccuPower® Dual-HotStart ™ RT-qPCR PreMix.
Experiment with HIV target. 10 fold serial dilution of Template RNA (1010 copies ~ 10 copies spiked in Human Total RNA).