MagListo™cfDNA Extraction Kit is designed for accurate and rapid extraction of cell-free DNA from various samples such as serum, urine, saliva using magnetic nano-bead and MagListo™ Magnetic Separation Rack. The magnetic separation allows easier isolation and purification of cell-free DNA from body-fluid compared to spin-column method.
| Reagent | K-3619M |
| CL buffer | 110 ml x 1 ea |
| CB buffer | 55 ml x 1 ea |
| CW1 buffer | 55 ml x 1 ea |
| W2 buffer | 80 ml x 1 ea |
| EA buffer | 25 ml x 1 ea |
| Magnetic Nano Bead-DNA | 1.8 ml x 6 ea |
| Proteinasa K | 25 mg x 2 ea |
| Sample Type | Mini | Midi | Maxi |
| Plasma / Serum /Urine / Saliva | ~ 1 ml | ~ 3 ml | ~ 5 ml |
| Elution volume | 30 ul | 50 ul | 70 ul |
PCR, Real-time PCR, Digital PCR, Next-generation sequencing (NGS)
Figure 1. cfDNA extracted from EDTA human plasma, human urine and human saliva. Samples were mixed with 25/200 bp Mixed DNA Ladder and purified using MagListo™ cfDNA Extraction Kit
M: Bioneer 25/100bp mixed ladder (Cat. No. 20-D-1020)
a) 1-3: cfDNA of plasma mixed Bioneer ladder purified with Bioneer MagListo™ Kit
b) 1-3: cfDNA of urine mixed Bioneer ladder purified with Bioneer MagListo™ Kit
c) 1-3: 1-3: cfDNA of saliva mixed Bioneer ladder purified with Bioneer MagListo™ Kit
Frigure 2. cfDNA from EDTA human plasma, human serum purified with MagListo™ cfDNA Extraction Kit
M: Bioneer 25/100bp mixed ladder (Cat. No. 20-D-1020)
a) cfDNA of plasma mixed Bioneer ladder purified with Bioneer MagListo™ cfDNA Extraction Kit
b) cfDNA of serum purified with Bioneer MagListo™ cfDNA Extraction Kit
Bioneer is the holder of Quality Management System Certificates for the following standards.
E-mail : sales@bioneer.com
v0.0 / 2019-05-28
