Overview
SHIVD, also known as ‘Infection with Decapod iridescent virus 1 (DIV1)’, is a viral disease that is mortal to shrimps. When infected, it changes the color of shrimps. Their heads turn white, while their bodies turn red. Also, their outer shell gets weakened, which causes them to sink and ultimately leads to death. The mortality rate of SHIVD is about 80%, severely reducing shrimp production and causing massive economic damage to shrimp farmers. Hence, it is important to monitor and diagnose the disease to prevent the spread of SHIVD infection.
Features and Benefits
- Nested PCR
Detect SHIVD with nested PCR consisting of SHIVD 1st PCR Kit and SHIVD 2nd PCR Kit.
- Convenience & Reproducibility
All reactants necessary for PCR including primers are lyophilized in each PCR tube, providing reproducible results in a convenient way.
- Sensitivity
By applying the patented PyroHotStart(Enzyme-mediated HotStart) technology that minimizes non-specific reactions and maximizes reaction efficiency, only the target gene can be effectively amplified even with a trace amount of template DNA.
- Stability
Included stabilizer in the PCR reaction mixture provides increased stability compared to solution-type products.
▶ Specification
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Type
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PreMix
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Target
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Shrimp Hemocyte Iridescent Virus (SHIVD)
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Instrument
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Thermal Cycler
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Specimen
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Environmental sample etc.
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Tests per kit
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96 tests
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▶ Experimental Data
Figure 1. SHIVD detection experiment result.
AccuPower® SHIVD PCR Kit was used, and 5 µl PCR reaction product was electrophoresed on 1.5% TBE Agarose gel.
◈ Manual
• Manual_AccuPower® SHIVD PCR Kit
◈ MSDS
• MSDS_K-2921 AccuPower® SHIVD PCR Kit EN ver.0
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate
