AccuPower® RocketScript™ RT Master Mix (2X, 1ml)

Features and Benefits

• Thermostable Activity

  Excellent thermal resistance of RocketScript ™ RTase active even at 70℃, making it suitable for cDNA synthesis of secondary RNA structure with comprehensive selection of compatible temperature ranging from 42 to 70 ℃ depending on the required experimental condition and effective cDNA synthesis 

  

• Reproducibility

  Mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results

• Easy of use

  Simplified protocol without the need of template RNA incubation at 70 degrees, allowing anyone to easily synthesize RNA with a large secondary structure

Application

• First-strand synthesis of cDNA from RNA molecules (RT)

• Random priming reaction

• cDNA library construction

• Probe labeling

• mRNA 5’-end mapping by primer extension analysis

• PCR

• Real time PCR

Experimental data

Figure. Sensitivity comparison between AccuPower^® RocketScript ™ RT Premix and M-MLV RTase
Sensitivity results of AccuPower^® RocketScript ™ RT PreMix using GAPDH compared with conventional Reverse transcriptases.
Each 100 ng – 10 fg of total RNA used for RT and the same amount of RT products used for electrophoresis.

Lane 1: 10 fg Human total RNA from HeLa cell
Lane 2: 100 fg Human total RNA from HeLa cell
Lane 3: 1 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell
Lane 5: 100 pg Human total RNA from HeLa cell
Lane 6: 1 ng Human total RNA from HeLa cell
Lane 7: 10 ng Human total RNA from HeLa cell
Lane 8: 100 ng Human total RNA from HeLa cell