AccuPower® PCR Master Mix (2X, 10 ml)

The AccuPower^® PCR Master Mix contains DNA Polymerase, dNTP and reaction buffer in a 2xMaster mixed solution format. Patented chemical stabilizer maintains the activity of the 2xMaster mixture for over 1 years in the freezer.

※ This product is shipped in dry ice.

$366.00

Cat. No.

K-2018-1

Product Overview

Features and Benefits

Stability
Addition of stabilizing agents allows delivery at room temperature and enhanced stability compared to solution-type products
User-friendly

Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests simply by adding template DNA and primers
Sensitivity
Excellent sensitivity and amplification efficiency even with small amounts of DNA
Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables

Application

• Conventional PCR

• Primer extension

• TA cloning

• Gene sequencing

Experimental data

Figure. Comparison of heat-stability of AccuPower^® PCR PreMix

AccuPower^® PCR PreMix is incubated at 95℃ with various time.
Kit: AccuPower^® PCR PreMix (Batch-1/2)

A; 30 min, B; 60 min, C; 90 min

Lane 1; Human genomic DNA 10 ng
Lane 2; Human genomic DNA 1 ng
Lane 3; Human genomic DNA 100 pg
Lane 4; Human genomic DNA 10 pg
M ;100 bp plus DNA ladder (Bioneer Cat. No: D-1035)

Technical/Specs

▶ Specifications

Enzyme	Top DNA polymerase
5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment Size	Up to 10 kb

▶ Experimental data

Figure 1. High sensitivity of AccuPower^® PCR PreMix

Lane 1; Lamda DNA 10 ng Lane 2; Lamda DNA 1 ng
Lane 3; Lamda DNA 100 pg Lane 4; Lamda DNA 10 pg
Lane 5; Lamda DNA 1 pg Lane 6; Lamda DNA 100 fg
Lane 7; Lamda DNA 10 fg Lane 8; Lamda DNA 1 fg
Lane 9; Human genomic DNA 10 ng Lane 10; Human genomic DNA 1 ng
Lane 11; Human genomic DNA 100 pg Lane 12; Human genomic DNA 10 pg
M; 100 bp plus DNA ladder (Bioneer Cat. No: D-1035)

Figure 2.Comparison of PCR amplification sensitivity between AccuPower^® PCR PreMix from Bioneer and other suppliers‘ PCR kit.

Lane 1; Lamda DNA 10 ng
Lane 2; Lamda DNA 1 ng
Lane 3; Lamda DNA 100 pg
Lane 4; Lamda DNA 10 pg
Lane 5; Lamda DNA 1 pg
Lane 6; Lamda DNA 100 fg
Lane 7; Lamda DNA 10 fg
Lane 8; Lamda DNA 1 fg

Figure 3. Comparison of PCR amplification of long targets between AccuPower^® PCR PreMix from Bioneer and other supplier's PCR kit.

A; 5 kb B; 6 kb C; 7 kb

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat.No.	Product Description	Option				Price
K-2012	AccuPower^® PCR PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-strip tubes with attached cap	96 tubes	20 µl/rxn	$K-2012
K-2036					20 µl/rxn (-dye)	$K-2036
K-2013					50 µl/rxn	$K-2013
K-2016				480 tubes	20 µl/rxn	$K-2016
K-2037					20 µl/rxn (-dye)	$K-2037
K-2017					50 µl/rxn	$K-2017
K-2011			0.5 ml thin wall microtube	100 tubes	50 µl/rxn	$K-2011
K-2260-1			thin-wall 96-well flat plate		10 µl/rxn	$K-2260-1
K-2260-4			thin-wall 96-well flat plate		20 µl/rxn	$K-2260-4
K-2260-2			thin-wall 96-well full-skirted plate		10 µl/rxn	$K-2260-2
K-2260-5			thin-wall 96-well full-skirted plate		20 µl/rxn	$K-2260-5
K-2260-3			thin-wall 96-well semi-skirted plate		10 µl/rxn	$K-2260-3
K-2260-6			thin-wall 96-well semi-skirted plate		20 µl/rxn	$K-2260-6
K-2080-1			thin-wall 384-well full-skirted plate		5 µl/rxn	$K-2080-1
K-2080-2					10 µl/rxn	$K-2080-2
K-2080-3					20 µl/rxn	$K-2080-3
K-2018		MasterMix type	1 ml of 2X Master mix solution	1 ml * 1 tubes		$K-2018
K-2018-1		MasterMix type	10 ml of 2X Master mix solution	1 ml * 10 tubes		$K-2018-1