▶ Components
• 10X reaction buffer with (or without) MgCl2 : Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
• Dilution buffer : 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH8.0
• 10 mM dNTPs mix: 2.5 mM of each dNTP
▶ Concentration
250 Units (5 U/μl)
▶ Storage Conditions
20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0
▶ Storage Temperature
-20℃
▶ Experimental data
Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript™ Cycle RT PreMix (Bioneer, Cat. No. K-2201) was used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.
Target: human GAPDH gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human total cDNA
Lane 2; 1 ng of human total cDNA
Lane 3; 100 pg of human total cDNA
Lane 4; 10 pg of human total cDNA
Figure 2. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.
Lane M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 2 kb fragment (human tumor protein p53 gene)
Lane 2; 3 kb fragment (human tumor protein p53 gene)
Lane 3; 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4; 8 kb fragment (human hemoglobin epsilon 1 gene
Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.
Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 11 kb fragment
Lane 2; 13.5 kb fragment
Lane 3; 17.6 kb fragment
Lane 4; 21.4 kb fragment
Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min.
PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.
Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 15 kb fragment
Lane 2; 20 kb fragment
Lane 3; 25 kb fragment
Lane 4; 30 kb fragment
