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ProFi Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2, 10 mM dNTPs (1,000 units)
![](https://eng.bioneer.com/media/catalog/product/cache/image/210x140/af097278c5db4767b0fe9bb92fe21690/e/-/e-2202_1.jpg)
ProFi Taq DNA polymerase is improved from traditional Taq DNA polymerase with higher efficiency, making it suitable for long-range PCR. Furthermore, this enzyme is compatible with various target genes such as complex genomic DNA or cDNA templates, and low-copy targets.
※This product is shipped in dry ice.
Features and Benefits
- Sensitivity
Higher amplification efficiency and sensitivity than other enzyme products by using lambda DNA and human gDNA as a template
- Long Range PCR
Effective amplification especially lambda DNA up to 30kb and human gDNA up to 21kb.
- Reproducibility
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system
Application
- Primer extension
- Long-range amplification from genomic DNA
- High amplification efficiency
- Excellent performance on difficult template
- Amplification of low-copy targets
- High yield and high sensitivity PCR
Experimental data
![](/images/products/accupower_ProFi_taq_f01.gif)
Figure. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec.
PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.
Target: human insulin receptor gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA