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Pfu DNA Polymerase, 10X Reaction Buffer, with 10 mM dNTPs (250 units)
Pfu DNA Polymerase is derived from Pyrococus furious (bacteria).
This product has 3' → 5' scale (proofreading) activity with a high fidelity reducing errors and an excellent specificity preventing formation of non-specific products.
※This product is shipped in dry ice.
$110.00
Cat. No.
E-2015-1
Features and Benefits
- High fidelity PCR
Proofreading activity with 3' → 5' exonuclease
- High specificity
Better specificity than Taq DNA polymerasde with reduced chance of non-specific products
- Terminal transferase activity
Guaranteed blunt-ended PCR product by blocking terminal transeferase activity
- Reproducibility
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system
Application
- Gene synthesis
- PCR or primer extension requested high fidelity
- Blunt-end PCR cloning or mutagenesis requested high fidelity
Experimental data
Amplication of limiting amounts of template DNA
Figure. Lambda DNA was amplified using 1 units of enzyme in 20 µl reaction volume.