The Future of Gene Synthesis
Megabase-Scale Capacity Enhanced by
AI Technology
30-yr+ accumulated experiences,
from Raw Materials to Gene Synthesis
All-in-One
Platform
Raw materials
Oligo Synthesizer
Fast & Cost Effective
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Gene assembly by BIONEER's new technology
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Enhanced efficiency in gene synthesis
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In-house processes from raw materials to Gene synthesis
Long gene synthesis
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We overcome the limitation of synthesizable gene length by Gibson assembly and Golden gate assembly
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Longer genes with shorter TAT and an affordable price
Quality Aassurance
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Free QC and re-shipment within 6 months
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Codon Optimization/Deoptimization based on 30-yr+ accumulated technical experience
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100% sequence guarantee by Automatic DNA sequencer (ABI 3730)
Order System
Contract Research (CRO) Services
From design to synthesis, analysis, and validation: CRO services tailored to your research objectives: Bioneer offers therapeutic oligonucleotide design, protein-design-led mRNA engineering, and protein engineering tailored to your research objectives. Our proprietary design platforms and collaboration across specialist departments support contract research programs that connect candidate design with synthesis, quality analysis, and experimental validation.
Therapeutic Oligonucleotide Design
From sequence design to chemical modification and validation for siRNA, ASO, and other therapeutic oligonucleotides: We support the design of a range of therapeutic oligonucleotides, including siRNA (RNAi) · ASO gapmer · SSO · ncRNA-directed silencing · saRNA (RNAa) · miRNA mimic · anti-miR (antagomir) · ADAR-recruiting editing oligo (AIMer · EON) · suppressor tRNA (ACE-tRNA) · CpG-ODN · aptamer conjugate (ApDC · AOC) · de novo aptamer scaffold, chemical modifications, and delivery requirements are assessed according to the target and mechanism of action. Finalized designs can be connected with oligonucleotide synthesis, quality analysis, and validation experiments.

Therapeutic Oligonucleotide Design Whitepaper

mRNA Engineering
We first design the protein to be expressed, then optimize the mRNA that encodes it: Protein candidates are first designed and selected to meet the research objectives. We then optimize codon usage, secondary structure, and untranslated regions (UTRs) of the encoding mRNA while preserving the selected amino acid sequence. Proprietary tools including GeneCrafter™, IVTDesigner™, and UTRDesigner™ support candidate designs that consider translation efficiency and stability. DNA template preparation, mRNA synthesis, quality analysis, and expression validation can be integrated within the agreed project scope. Depending on the research objectives, circular RNA (circRNA) and self-amplifying RNA design using CircularDesigner™ and SaRNADesigner™ can also be discussed.

Protein candidate design and selection → mRNA sequence, structure, and UTR optimization → DNA template and mRNA synthesis → Quality analysis and expression validation

Codon Optimization · mRNA Structure Optimization · UTR Design · Circular RNA Design · Self-Amplifying RNA Design

Protein Engineering
From variant, recombinant, and de novo protein design to expression, purification, and functional validation: Using the Unified Protein Engineering Platform™, we design protein variants, recombinant proteins, and de novo protein candidates to meet your research objectives. Computational assessment of structure, function, stability, solubility, and expression potential helps prioritize candidates for experimental evaluation. Through integration with the Gene-to-Protein service, projects can combine gene synthesis, protein expression and purification, quality analysis, and functional validation.

Protein Engineering Whitepaper · Research-Use Protein Purification Whitepaper

Consultation and Service Scope: The scope, deliverables, timeline, and pricing for specialist design and CRO services are defined through project-specific consultation. Once the design is finalized, the relevant in-house departments proceed with oligonucleotide synthesis and the supply of research-use-only (RUO) products. Upon request, these services can also be connected with contract research organization (CRO) services, including quality analysis and experimental validation. Close collaboration among our specialized teams enables coordinated support from design and synthesis through analysis and experimental validation, tailored to each customer's objectives and project requirements. For an effective consultation, please include your research objectives, target or available sequence, requested service area, and desired deliverables.

CRO Service Inquiries: Email geneorder@bioneer.co.kr

Gene Synthesis Custom Order
This service provides genes with 100% accuracy by only using the gene sequence information given by the customers. For those wishing for faster service time, please use our Rapid Gene Synthesis Service. For ordering DNA fragments, please use our AccuGeneBlock Service.

Enhanced sequence stability and synthesis success rates using our AI-driven GeneCrafter™(Whitepaper) codon optimization.

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Cloning Custom Order
This service provides cloning services for inserts provided by the customers. TA Cloning Services are also available. If you do not have an insert gene, please use our Gene Synthesis & Cloning Service.
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Mutagenesis Custom Order
This service provides gene with inner- or end-point mutations. If you do not have the template gene, please use our Gene Synthesis & Mutagenesis Service.
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mRNA Synthesis Custom Order
This service provides various types of mRNA such as 5’ capping and 3’ poly (A) tailing. If you do not have the template gene, you can use our Gene & Standard mRNA Synthesis or Gene & Complete mRNA Synthesis Service.
  • Experience high-quality mRNA with maximized expression and stability via IVTDesigner™(Whitepaper)'s sophisticated structural optimization.
  • Generate optimal, custom UTRs that push ribosome loading (RLS) and translation initiation efficiency to the absolute limit, leveraging UTRDesigner™(Whitepaper)'s advanced LBS (Lookahead Beam Search)-based de novo design technology (Coming Soon).
  • Design ciDesign circular RNAs (circRNA) that completely block splicing interference and maximize circularization yield, powered by CircularDesigner™(Whitepaper)'s sophisticated PIE system spacer design and P1 stem structure optimization algorithms. Furthermore, experience next-generation RNA therapeutics development with CircularDesigner™'s 450+ component library, 15 alphavirus replicon modules, and an integrated suite of 92 platforms (717+ presets). Key platforms include: ARTEMIS, TITAN, TITAN-H, NEXUS, PROMETHEUS, CALYPSO (huNoV triM-RdRp, 93.7% dsRNA reduction), OUROBOROS (7,000x protein efficiency without RdRp), HELICASE, ZENITH, NARNOS, CHIMERA (Twister P1 + HDV + RtcB), CRUCIBLE, EPIC, VORTEX, FORGE (dual ribozyme + T4 PNK + T4 Rnl2 splint ligation), TOMBUS, OURMIA, and virus-family platforms (FLAVI, BROME, ARTERIO, PICORNA, AQUA, POTEX); fungal-origin RdRp platforms (HETERO, HYPO, POLYMYCO, PARTITI, COLLETO, MAGNAPORTHE, USTILAGO, CURCUMA, BEAUVERIA, RIMOSA); tissue-targeted platforms (AETHER, CEREBRO, HEPATO, PULMO, CARDIO, NEPHRO, OCULUS, OSTEO); and application-specific payload platforms spanning veterinary medicine, dermatology, neuroscience, metabolic disease, antiviral/anti-fibrotic therapy, and enzyme replacement therapy. All components are FTO (Freedom to Operate)-verified public-domain elements (Software Development Completed).
  • Experience self-amplifying RNAs engineered to minimize innate immune responses and maximize replicon amplification efficiency through SaRNADesigner™(Whitepaper)'s proprietary CSE (Conserved Sequence Element) structural interference prediction and U-depletion technologies (Coming Soon).
Order
Gene to Protein Synthesis Custom Order
  • This service undergoes all the steps from gene synthesis to protein synthesis. N-term His tag & C-term His tag vectors can be selected. For pBT7-C-His vectors, please be aware of the stop codons.

    Maximize target protein expression efficiency through GeneCrafter™'s precise AI-based codon optimization.

  • Design protein variants, recombinations, and de novo proteins that balance thermostability, solubility, and activity Unified Protein Engineering Platform™ (Whitepaper) evaluates every candidate through a 17-stage integrated pipeline. 24 internal modules spanning structure prediction, molecular dynamics, and free energy calculation screen candidates across multiple axes, filtering out designs that will not express or will aggregate before you pay to synthesize them. Built-in immunogenicity prediction and freedom-to-operate (FTO) pre-screening reduce the risk that research stage results require rework at commercialization.
  • A research-use purification whitepaper covering expression host, tag placement, protease choice, polishing, and QC criteria across nine protein classes, from soluble enzymes to membrane proteins, antibodies, and complexes.
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Cutting-Edge Fragment Assembly Technology and Gene Synthesis Service
Fig. 1 Final construct
Fig. 2 Colony Screening
Fig. 3 Restriction enzyme mapping
The sample loading is in triplicate.
M: 100 bp Plus DNA Ladder (Bioneer D-1035)
Fig. 4 Sequencing for final validation
Fig. 1 Final construct
Fig. 2 Colony Screening and Junction PCR for Fragment Assembly Validation
M: 1 kb DNA Ladder (Bioneer D-1040)
Fig. 3 NGS for final validation
Gene Synthesis Rapid Gene Synthesis AccuGeneBlock
Delivery Form Plasmid DNA Plasmid DNA DNA Fragmant(PCR product)
Gene length From 1 bp 1-1000 bp 100-1000 bp
Accuracy
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Guaranteed exact plasmid DNA sequences with Bioneer's Gene synthesis service
Rapid
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Gene synthesis avaliable only within minimum of 5 days
AccuGeneBlock Service
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Quick and easy service providing DNA fragments of 1 Kbp or less in at least 7 days for synthetic biology research
Gene Synthesis
Gene Length (bp) Synthesis price(Normal sequence) Synthesis Time
Up to 400 $77 5-10 working days,
Average 7 working days
401-1,200 $0.19/bp 5-15 working days,
Average 9 working days
1,201-2,000 $0.19/bp 10-17 working days,
Average 12 working days
2,001-3,000 $0.19/bp 10-25 working days,
Average 16 working days
3,001-15,000 $0.19/bp 12-30 working days,
Average 20 working days
15,001-100,000 $0.19/bp Inquire
Over 100,001 From $0.19/bp Inquire
Delivery Form 1-2 µg of vacuum dried plasmid *Low copy plasmid is 0.5-1 µg
Cloning Vector pBHA Basic vector
Other (bioneer vector) $50.00 (+Add 5 working days)
Subcloning Price $200.00 (If ordered with commercial or custom vector of your choice)
Plasmid Increase Service
*Low copy plasmid (Inquire)		 4 μg 100 μg 300 μg Over 500 μg
$23 $54 $154 Inquire
* Price and delivery period are raised for gene segments containing complex structures such as high or low GC content, repeat sequences or homopolymeric runs
* If you want to use subcloning with commercial vector, your order will be continued to Gene Cloning Service.
* Only the synthesized genes with 100% sequence match will be shipped after the sequencing procedure
* Genes that produces toxic substances or inhibits growth in E.coli cell system will be delivered in the form of PCR products or plasmid DNA, even during the sequence check during the synthesis process.
* If the gene synthesis is ordered for the protein expression or purification, Bioneer's ExiProgen™ service can be used to directly obtain the proteins. (Go to ExiProgen™ page for more details )


* Order Cancellation Policy:
50% of the total charge will be billed if the service is cancelled within 5 days
80% of the total charge will be billed if the service is cancelled after 5 days
No additional fee will be charged after the guarantee period, the period for completing gene synthesis service (except the delivery time)
Vector Map Sequence Vector Map Sequence Vector Map Sequence
High Copy pBHA View Low Copy pBLA View Expression pBT7-N-His View
pBHK View pBLK View pBT7-C-His View
pBHC View pBLC View pBT7-N-GST View
pBHZ View pBLZ View pBT7-C-GST View
In-vitro
Transcription		 pBIC-A View
AccuGeneBlock Service
Synthesis price 100-500 bp $96
501-1,000 bp $96 ( + $19/100 bp)
Synthesis period 100-500 bp 7 working days
501-1,000 bp 10 working days
Delivery Form 0.5-1 µg of lyophilized PCR product
Additional Service Increased Quantity 5 µg/$77
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Synthesis may be limited if the gene contains high or low GCs, repeat sequences, or homo-polymeric runs. (Please use Gene synthesis service)
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100-1,000 bp gene can be synthesized.
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*PCR product is provided with 3'A-tailing, but blunt ends can be also selected.
Rapid Gene Synthesis Service
Synthesis period 1-500 bp 5 working days
501-1,000 bp 8 working days
Synthesis price Inquire
Delivery Form 1-2 µg of vacuum dried plasmid
Cloning Vector pBHA (Basic vector)
Plasmid
Increase Service
*Low copy plasmid
(Inquire)		 4 μg 100 μg 300 μg Over 500 μg
$23 $54 $154 Inquire
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Synthesis containing complex structures such as high or low GC, repeated sequences, homo-polymeric runs, etc. may be limited. (General Gene Synthesis service available)
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Genes synthesis that inhibit growth or contain toxicity to E. coli cell system may be limited. (General Gene Synthesis service available), We do not guarantee the synthesis period if confirmed during synthesis.
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If the synthesis is completed after the guarantee period, 40% of discount will be applied to the estimated price.
Consultation
• Consultation service
Tel. +82-42-930-8777(Customer Support Center) or +82-42-930-8793, 8515 (Synthetic Biology Team)
• Consultation time
Weekdays from 9:00 am to 6:00 pm (closed on weekends and holidays)
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CUSTOMER CENTER
1588-9788
Bioneer Global Center, 71, Techno 2-ro, Yuseong-gu, Daejeon 34013, Republic of Korea   
TEL : +82-42-939-6333
FAX : +82-42-939-6444
Business Registration Number : 301-81-27217