▶ Specifications
• Target-specific primer design using primer blast and Bioneer’s bioinformatics tools
• Elimination of self primer-dimer artifacts
• Generation of single peak in the dissociation curve
• Generation of single band in the gel-electrophoresis
• Short amplicon size of 70 ~ 150 bp
Figure 1. Primer validation result (Example. RPL13A gene)
▶ Wide linear dynamic range & high PCR efficiency
• Wide amplification range of 102 ~ 107 copies
• qPCR amplification efficiency of 90 ~ 110% in compliance with the MIQE Guide line
1) PCR efficiency test
: An efficiency test of a purified qPCR product with 10 fold dilutions
Figure 2. [BIONEER] Dynamic range & PCR efficiency (Example. GAPDH gene).
It corresponds to the qPCR efficiency range recommended by the MIQE guideline (100%±10%).
Limit of detection (LOD) is found at 100 copies or less.
Figure 3. [Competitor] Dynamic range & PCR efficiency (Example. GAPDH gene).
It corresponds to the qPCR efficiency range recommended by the MIQE guideline (100%±10%).
An irregular gap was observed between 103 and 102 copies, showing limit of detection (LOD) at 103 copies or less. This implies that its detection efficiency is lower than our company's products.
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2) Target specificity test
: A 10 fold Serial dilution of purified qPCR product + efficiency test of reference mouse cDNA (1㎍ mouse total RNA/20㎕).
Figure 4. Target specificity test using [BIONEER] Mouse template (Example. ACTB gene).
It can be seen that specificity for the target gene and qPCR efficiency are within the range recommended by the MIQE guideline (100%±10%).
Limit of detection (LOD) is found at 100 copies or less.
Figure 5. Target specificity test using [Competitor] Mouse template (Example. ACTB gene).
Aggregation is found in the efficiency fluorescence curve from 104 copy.
The efficiency is 161%, which is out of the qPCR efficiency range recommended by the MIQE guideline (100%±10%). This implies that its target specificity is lower than that of our company's.
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