dC(Ac)-CE phosphoramidite

• The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is treatment with concentrated ammonia for 8 hours at 55°C or by the use of methylamine gas.

  (However, it is recommended to use dC-Ac(CE)-PA because dC(Bz)-CE-PA can be acetylated when methylamine gas is used)

• The purities of manufactured nucleoside phosphoramidites are determined by ³¹P-NMR and RP-HPLC analysis and controlled to be greater than 99%.

• All reagents are conveniently packaged for ABI instruments. Expedite- and Mermade-compatible vials are also available upon request.

Figure 1. HPLC data : ≥ 99%	Figure 2. 31P-NMR data : ≥ 99%