T4 DNA ligase catalyzes the formation of phosphodiester bonds between the 5'-phosphate terminal and the 3'-hydroxyl terminal of the DNA strand to repair not only single strand breaks in duplex DNA, RNA or DNA / RNA hybrids, but also to connect blunt-ends and cohesive-end ends.
※This product is shipped in dry ice.
DNA ligations in 5 minutes for cohesive end and in 10 minutes for blunt end DNA at 25°C
Suitable for all DNA ligation
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
Figure. Ligation test
- Lane 1: DNA fragment (digested with EcoR V)
- Lane 2, 3, 4, 5: T4 DNA Ligase 100 U, 16°C, 10, 20, 30 and 60 minutes
- Lane 6, 7, 8, 9: T4 DNA Ligase 100 U, 25°C, 10, 20, 30 and 60 minutes
- Lane 10, 11, 12,13: T4 DNA Ligase 100 U, 37°C, 10, 20, 30 and 60 minutes
- Lane 14: Lambda DNA (digested with Hind III)
- Lane 15, 16, 17: T4 DNA Ligase 200 U, 16°C, 10, 20 and 30 minutes
- Lane 18, 19, 20: T4 DNA Ligase 200 U, 25°C, 10, 20 and 30 minutes
- Lane 21, 22, 23: T4 DNA Ligase 200 U, 37°C, 10, 20 and 30 minutes
