Customized AccuPower® PCR/RT/qPCR PreMix Service

Custom AccuPower® PCR PreMix Service provides tailor-made PCR products to your PCR characteristics for highly reproducible PCR tests.

Customized PCR/RT/qPCR PreMix order form

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Cat. No.
CUSTOM-PREMIX

Overview

Products made from our Custom AccuPower® PCR PreMix Service have great advantage of high reaction specificity and PCR amplification efficiency, along with minimized amplified products from non-specific reactions and primer-dimer formation. Premix for various types of PCR reactions can be ordered such as complex genomics, cDNA templates, low-copy targets, multiplex PCR, and etc. Moreover, all the requested reactants essential for the hot start PCR such as DNA polymerase, dNTPs, reaction buffers, and thermostable PPase are mixed with the ordered primer sets and vacuum-dried, each packaged with amounts sufficient for one run to start the PCR reaction conveniently just by adding the template and the D.W.

Features and Benefits

  • Stability

    Maintaince of enzyme activity for a month at room temperature, a year in a freezer through the use of a patent stabilizer 

  • Specificity

    Simplified procedure with all components necessary for PCR reaction packaged mixed with the inquired primer set in each PCR tube for the reaction to be performed immediately by adding DNA and D.W only., along with tracking dyes and precipitants required for electrophoresis included to eliminate the need of sample loading buffer and minimize carry-over contamination by aerosol generation

  • Reproducibility

    Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product

Experimental data

Figure. Custom AccuPower® PCR PreMix: Reproducibility & Sensitivity. Custom AccuPower® DsRed2 HotStart PCR PreMix was tested three times, A-1, A-2 and A-3, with the condition of: 1. 94°C 2 min; 2. 30 cycles of 94°C 30 sec, 58°C 30 sec and 72°C 30 sec; 3. 72°C 3 min. 5 ul of amplified PCR product was run on a 2% TBE agarose gel.

Lane 1; 20-2x positive DNA. Lane 2; 20-3x positive DNA, Lane 3; 20-4x positive DNA, 
Lane 4; 20-4x positive DNA, Lane N; Negative control, Lane M; 100bp DNA Ladder (D-1030)

Customers

  Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA, Korea)
  Korea Centers for Disease Control and Prevention (Korea)
  Korea National Institute of Health (Korea)
  Chungnam National Institute of Public Health and Environment (Korea)
  The National plant quarantine service (Korea)
  National Agricultural Products Quality Management Service (Korea)
  National Fishery Products Quality Management Service (Korea)
  Research Center for Risk of LMO in Pukyong National University (Korea)
  Research Center for Resistant Cells in Chosun University (Korea)
  PKPORK (Korea)
  Seoul Song Do Colorectal Hostpital (Korea)
  Purigo company (Taiwan)
  Hylab company (Israel)
  Kirkhouse company (U.K) etc..

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