Overview
Products made from our Custom AccuPower® PCR PreMix Service have great advantage of high reaction specificity and PCR amplification efficiency, along with minimized amplified products from non-specific reactions and primer-dimer formation. Premix for various types of PCR reactions can be ordered such as complex genomics, cDNA templates, low-copy targets, multiplex PCR, and etc. Moreover, all the requested reactants essential for the hot start PCR such as DNA polymerase, dNTPs, reaction buffers, and thermostable PPase are mixed with the ordered primer sets and vacuum-dried, each packaged with amounts sufficient for one run to start the PCR reaction conveniently just by adding the template and the D.W.
Features and Benefits
- Stability
Maintaince of enzyme activity for a month at room temperature, a year in a freezer through the use of a patent stabilizer
- Specificity
Simplified procedure with all components necessary for PCR reaction packaged mixed with the inquired primer set in each PCR tube for the reaction to be performed immediately by adding DNA and D.W only., along with tracking dyes and precipitants required for electrophoresis included to eliminate the need of sample loading buffer and minimize carry-over contamination by aerosol generation
- Reproducibility
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product
Experimental data
Figure. Custom AccuPower® PCR PreMix: Reproducibility & Sensitivity. Custom AccuPower® DsRed2 HotStart PCR PreMix was tested three times, A-1, A-2 and A-3, with the condition of: 1. 94°C 2 min; 2. 30 cycles of 94°C 30 sec, 58°C 30 sec and 72°C 30 sec; 3. 72°C 3 min. 5 ul of amplified PCR product was run on a 2% TBE agarose gel.
Lane 1; 20-2x positive DNA. Lane 2; 20-3x positive DNA, Lane 3; 20-4x positive DNA,
Lane 4; 20-4x positive DNA, Lane N; Negative control, Lane M; 100bp DNA Ladder (D-1030)
Customers
Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA, Korea)
Korea Centers for Disease Control and Prevention (Korea)
Korea National Institute of Health (Korea)
Chungnam National Institute of Public Health and Environment (Korea)
The National plant quarantine service (Korea)
National Agricultural Products Quality Management Service (Korea)
National Fishery Products Quality Management Service (Korea)
Research Center for Risk of LMO in Pukyong National University (Korea)
Research Center for Resistant Cells in Chosun University (Korea)
PKPORK (Korea)
Seoul Song Do Colorectal Hostpital (Korea)
Purigo company (Taiwan)
Hylab company (Israel)
Kirkhouse company (U.K) etc..
▶ Specifications
|
Target
|
Human / Veterinary / Plant / Fish / Microorganism (Fungi, E.coli) etc
|
|
Instrument
|
AllInOneCycler™ 96 Gradient Thermal Block or other thermal block
|
|
Specimen
|
Taken from brain, cardiovascular, bone marrow, liver, lung, peritoneum, feces, septicemia, or other organs
|
|
Tests per kit
|
96
|
▶ Custom AccuPower® Products with PCR PreMix Service
ILivestock Related Products
• Custom AccuPower® PCV2 HotStart PCR PreMix
• Custom AccuPower® PRRS HotStart RT-PCR PreMix
• Custom AccuPower® PRRS Nested HotStart PCR PreMix
• Custom AccuPower® Q-Fever HotStart PCR PreMix
• Custom AccuPower® Brucella HotStart PCR PreMix
• Custom AccuPower® Cva A/B, fyuA, FIMC 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® Vat, iucD, Irp2 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® Tsb, Iss, astA 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® ConOri, CsPcSC43528 & STM3664 3-plex HotStart PCR PreMix
• Custom AccuPower® CPA, CPB, CPE & CPI 4-plex HotStart PCR PreMix
• Custom AccuPower® E. Coli EAST1 HotStart PCR PreMix
• Custom AccuPower® E. Coli PAA HotStart PCR PreMix
• Custom AccuPower® E. Coli AIDA-1 HotStart PCR PreMix
• Custom AccuPower® Lowsonia HotStart PCR PreMix
• Custom AccuPower® Brachyspira HotStart PCR PreMix
• Custom AccuPower® HPS, APP & A.Suis 3-Multiplex HotStart PCR PreMix
• Custom AccuPower® tcdB HotStart PCR PreMix
• Custom AccuPower® RVD1 HotStart RT-PCR PreMix
• Custom AccuPower® RVD2 HotStart RT-PCR PreMix
• Custom AccuPower® JEE HotStart RT-PCR PreMix
• Custom AccuPower® Salmonella HotStart PCR PreMix
• Custom AccuPower® EAE HotStart PCR PreMix
• Custom AccuPower® Malaria rVIV HotStart PCR PreMix
• Custom AccuPower® Clostridium pefrigens HotStart PCR PreMix
• Custom AccuPower® Malaria rPLU5/6 HotStart PCR PreMix
• Custom AccuPower® cdtA HotStart PCR PreMix
• Custom AccuPower® cdtB HotStart PCR PreMix
• Custom AccuPower® tcdA HotStart PCR PreMix
• Custom AccuPower® Neospora HotStart PCR PreMix
• Custom AccuPower® Toxoplasma HotStart PCR PreMix
• Custom AccuPower® Leptospra HotStart PCR PreMix etc.
IIGMO & Plant Related Products
• Custom AccuPower® P. destructiva HotStart PCR PreMix
• Custom AccuPower® P. exigua HotStart PCR PreMix
• Custom AccuPower® P. glomerata HotStart PCR PreMix
• Custom AccuPower® P. loticola HotStart PCR PreMix
• Custom AccuPower® P. medicaginis HotStart PCR PreMix
• Custom AccuPower® CMM5/6 HotStart PCR PreMix
• Custom AccuPower® CaMv p35s Multiplex PCR PreMix
• Custom AccuPower® NOS Multiplex PCR PreMix
• Custom AccuPower® DsRed Multiplex PCR PreMix
• Custom AccuPower® PB3/4 HotStart PCR PreMix etc.
IIIFish Related Products
• Custom AccuPower® White spot syndrome virus(146F1/R1) HotStart PCR PreMix
• Custom AccuPower® White sopt syndrome virus(146F1/R2) HotStart PCR PreMix
• Custom AccuPower® SVCV F1/R1 HotStart PCR PreMix
• Custom AccuPower® SVCV F1/R4 HotStart PCR PreMix etc.
IVOthers
• Custom AccuPower® D8M309 HotStart PCR PreMix
• Custom AccuPower® D11M5 HotStart PCR PreMix
• Custom AccuPower® D13M78 HotStart PCR PreMix
• Custom AccuPower® D14M14 HotStart PCR PreMix
• Custom AccuPower® D15M226 HotStart PCR PreMix
• Custom AccuPower® D18M64 HotStart PCR PreMix
• Custom AccuPower® D19M19 HotStart PCR PreMix
• Custom AccuPower® DXM210 HotStart PCR PreMix
• Custom AccuPower® BID HotStart PCR PreMix
• Custom AccuPower® rPLU 5/6 (1st)HotStart PCR PreMix
• Custom AccuPower® rVIF F/R (2nd) HotStart PCR PreMix
▶ Experimental data
• Custom AccuPower® Hotstart PCR PreMix
Custom AccuPower® Hotstart PCR PreMix is manufactured / produced by the customer's manufacturing method and Bioneer Premix patent, and the optimal sensitivity test is completed.
| Target |
E.Coli PAA |
Lawsonia |
Leptospira |
Neospora |
PM-Pasteurella |
| PCR |
95°C 15 min.
94°C 30 s/55°C
30 s/72°C 1 m
(30cy)
72°C 5 min |
95°C 15 min.
94°C 30 s/55°C
30 s/72°C 1 m
(30cy)
72°C 5 min |
94°C 10 min.
94°C 60 s/55°C
60 s/72°C 2 m
(30cy)
72°C 5 min |
95°C 15 min.
94°C 60 s/55°C
60 s/72°C 30 s
(30cy)
72°C 5 min |
95°C 15 min.
94°C 30 s/55°C
30 s/72°C 1 m
(30cy)
72°C 5 min |
| Size |
180 bp |
655 bp |
285 bp |
350 bp |
460 bp |
| Remarks |
Animal disease standard test |
Proceed to PM condition in animal disease standard test method |
Animal disease standard test |
Animal disease standard test |
Animal disease standard test |
| Target |
Actinobacillus
pleuropneumoniae |
Arcano
pyogenes |
Brachyspira hyo-
dysenteriae (Hyo) |
Streptococcus
suis |
| PCR |
95°C 15 min.
94°C 30 s/55°C
30 s/72°C 1m
(30cy)
72°C 5 min |
94°C 10 min.
94°C 45 s/55°C
45 s/72°C 60 s
(30cy)
72°C 5 min |
94°C 15 min.
94°C 30 s/62°C
30 s/72°C 60 s
(40cy)
72°C 5 min |
94°C 5 min.
94°C 60 s/55°C
60 s/72°C 60 s
(35cy)
72°C 5 min |
| Size |
422 bp |
655 bp |
320 bp |
688 bp |
| Remarks |
Animal disease standard test |
Literature |
Animal disease standard test |
Animal disease standard test |
| Target |
Rabies
(P:target gene) |
Rabies
(N:target gene) |
Brucella(16s) |
Toxoplasma
(B1 gene) |
| PCR |
42°C 15 min.
95°C 15 min.
94°C 30 s/50°C
30 s/72°C 30 s
[42(45)cy]
72°C 5 min |
42°C 15 min.
95°C 15 min.
94°C 30 s/50°C
30 s/72°C 30 s
[42(45)cy]
72°C 5 min |
95°C 5 min.
95°C 30 s/54°C
90 s/72°C 90 s
(35cy)
72°C 6 min |
94°C 4 min.
94°C 60 s/52°C
60 s/72°C 60 s
(35cy)
72°C 8 min |
| Size |
580 bp |
181 bp |
905 bp |
501 bp |
| Remarks |
- |
- |
Animal disease standard test |
Animal disease standard test |
| Target |
E.Coli ( EAST1 ) |
E.Coli ( AIDS-I ) |
E.Coli ( EAE ) |
| PCR |
95°C 5 min.
94°C 30 s/55°C
30 s/72°C 60 s
(30)cy
72°C 5 min |
95°C 5 min.
94°C 30 s/55°C
30 s/72°C 60 s
(30)cy
72°C 5 min |
95°C 5 min.
94°C 30 s/57°C
30 s/72°C 50 s
(35)cy
72°C 5 min |
| Size |
125 bp |
585 bp |
790 bp |
| Remarks |
Animal disease standard test |
Animal disease standard test |
Animal disease standard test |
| Target |
Staphylococcus
aureus
(16SrRNA,clfA) |
Campylobacter
(spp, C.coli) |
Enterococci(E.faecium,
E.faecalis) |
Swine
Mycoplasma |
| PCR |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35)cy
72°C 10 min |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35)cy
72°C 10 min |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35)cy
72°C 10 min |
95°C 15 min.
94°C 60 s/55°C
60 s/72°C 1 m 30 s
(35)cy
72°C 5 min |
| Size |
791 bp & 638 bp |
875 bp & 462 bp (C.coli) |
650 bp(E.faecium) &
941 bp(E.faecalis) |
948 bp & 346 bp |
| Remarks |
- |
- |
- |
Animal disease standard test |
• Custom AccuPower® Multiplex PCR PreMix
Custom AccuPower® Multiplex PCR PreMix is manufactured / produced by the customer's manufacturing method and Bioneer Premix patent, and the optimal sensitivity test is completed.
| Target |
Abortion and stillbirth inducing disease in cow I (Yersinia spp,
Campylobacter, Listeria) |
Abortion and stillbirth inducing disease in cow II
(Q-fever,
Chlamydophila, Leptospira) |
| PCR condi. |
95°C 5 min.
95°C 30 s/60°C
30 s/72°C 40 s
(35 cy)
72°C 5 min |
95°C 5 min.
95°C 30 s/65°C
30 s/72°C 40 s
(35 cy)
72°C 5 min |
| Size |
234, 365 & 594 (bp) |
289, 357 & 414 (bp) |
| Remarks |
Animal disease standard test |
Animal disease standard test |
| Target |
Staphylococcus
aureus
(16SrRNA,clfA) |
Campylobacter
(spp, C.coli) |
Enterococci(E.faecium,
E.faecalis) |
Swine
Mycoplasma |
| PCR |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35cy)
72°C 10min |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35cy)
72°C 10 min |
95°C 15 min
(adjustment).
94°C 60 s/55°C
60 s/72°C 60 s
(35cy)
72°C 10 min |
95°C 15 min.
94°C 60 s/55°C
60 s/72°C 1 m 30 s
(35cy)
72°C 5 min |
| Size |
791 bp & 638 bp |
875 bp & 462 bp (C.coli) |
650 bp (E.faecium) &
941 bp (E.faecalis) |
948 bp & 346 bp |
| Remarks |
- |
- |
- |
Animal disease standard test |
| Target |
Astro-Sapo RT-PCR kit |
| PCR |
48°C 40 min,
95°C 15 min,
95°C 30 s/58°C
30 s/72°C 1 min
(35cy)
72°C 7 min |
| Size |
449 bp & 719 bp
(109 ~ 103 Copy) |
| Remarks |
KNIH |
|
| PRRS RT-PCR kit |
50°C 30 min,
95°C 15 min,
94°C 30 s/55°C
30 s/72°C 40 s
(35cy)
72°C 10 min |
433 bp
(K university offer RNA) |
| |
|
◈ MSDS
• MSDS_Custom AccuPower® PreMix
◈ Brochure
• Custom AccuPower® PreMix
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 – certificate
• EN ISO 13485 - certificate
▶ Customization Service Condition Chart
| Minimum Quantity |
1920 tube |
| Production Period |
Single Custom PreMix |
~ 10 business days |
| 2~3-Multiplex Custom PreMix |
~12 business days |
| 4-Multiplex Custom PreMix |
~16 business days |
| 5-Multiplex Custom PreMix |
Inquire |
When the template is not provided, the production period will be longer.
Client verification period is excluded from production period.
The fee for oligo synthesis will be separately added to the quoted price.
