Primer & Probe Design Service
Primer & Probe Design Service designs primer and probe set suitable for your experimental purpose. By providing the genetic information of desired species (human, mouse, rat, dog, etc.), we can not only design them for conventional PCR, but also dsDNA binding dye(GreenStar™) type and hydrolysis probe type for real-time PCR.
As PCR primer sets can greatly influence the result, those designs must be optimized and tested before using the primers. This service can provide a primer set that shows the target specific amplification and low dimer formation chance using the most updated information on genetics listed on NCBI database RefSeq (Reference Sequence) by using our rich experiences on the primer and the probe designs.
Features and Benefits
- Professional Design
Customized primer design designed by considering all the essential conditions that the customers need such as such as target specificity, Tm value, and PCR product sizes
- Accurate & Economical
Primers validated with target-specific amplification test through algorithm and BLAST mechanism, reducing the time and cost by mis-designed primer sets.
- Bioneer Guarantee
Warranty service providing free additional design if the primer set shows insufficient or non-specific amplification due to design problems
Optimization of qPCR primer and probe
The target-specific amplification of PCR primers and probe sequences provided were tested through algorithms and BLAST. However, non-specific amplifications and dimers may be formed due to external factors such as PCR instruments, reagents, and experimental conditions. Therefore, it is highly recommended to use primer and probes after checking the compatibility with your conditions to get accurate results.