Primer & Probe Design Service

Primer & Probe Design Service designs primer and probe set suitable for your experimental purpose. By providing the genetic information of desired species (human, mouse, rat, dog, etc.), we can not only design them for conventional PCR, but also dsDNA binding dye(GreenStar™) type and hydrolysis probe type for real-time PCR.

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Cat. No.
S-6000

Overview

As PCR primer sets can greatly influence the result, those designs must be optimized and tested before using the primers. This service can provide a primer set that shows the target specific amplification and low dimer formation chance using the most updated information on genetics listed on NCBI database RefSeq (Reference Sequence) by using our rich experiences on the primer and the probe designs.

Features and Benefits

  • Professional Design

    Customized primer design designed by considering all the essential conditions that the customers need such as such as target specificity, Tm value, and PCR product sizes

  • Accurate & Economical

    Primers validated with target-specific amplification test through algorithm and BLAST mechanism, reducing the time and cost by mis-designed primer sets.

  • Bioneer Guarantee

    Warranty service providing free additional design if the primer set shows insufficient or non-specific amplification due to design problems

Note

Optimization of qPCR primer and probe

The target-specific amplification of PCR primers and probe sequences provided were tested through algorithms and BLAST. However, non-specific amplifications and dimers may be formed due to external factors such as PCR instruments, reagents, and experimental conditions. Therefore, it is highly recommended to use primer and probes after checking the compatibility with your conditions to get accurate results. 

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