Features and Benefits
-
Flexible sample choice
Isolation of RNA from a wide variety of samples such as cultured cells, animal tissues, plant tissues, etc.
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High binding capacity
Up to 120 µg using silica based RNA binding columns
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Minimized contamination
Columns Individually sterilized and packaged
Application
• cDNA synthesis
• RT-PCR
• Quantative real-time RT-PCR
• Northern blot analysis
▶ Specifications
| Column loading volume |
800 μl |
| Max binding capacity |
~ 120 μg |
| Elution volume |
30 μl - 100 μl |
| Preparation time |
~ 20 min |
| Sample |
Amount |
RNA Yield |
Purity |
| Cultured cells |
104 – 108 cells |
15 – 20 μg |
A260/280 > 2.0
A260/230 > 1.7 |
| Mammalian tissue |
25 – 50 mg |
10 - 60 μg |
| Plant tissue |
100 mg |
30 - 60 μg |
* Differences in the quantified value may occur depending on the types of samples.
▶ Components
| Components |
K-3140G
|
K-3141G |
| RB Buffer (RNA Binding) |
60 ml x 1 ea |
30 ml x 1 ea |
| RWB1 Buffer (1st Washing) |
72 ml x 1 ea |
36 ml x 1 ea |
| RWB2 Buffer (2nd Washing) |
21 ml x 2 ea |
21 ml x 1 ea |
| ER Buffer (Elution) |
20 ml x 1 ea |
10 ml x 1 ea |
| AccuPrep® Binding Column-Ⅲ |
100 ea |
50 ea |
| 1.5 ml Tube (for collection) |
100 ea |
50 ea |
| User's Guide |
1 ea |
1 ea |
▶ Experimental data
Figure 1. Cultured cell RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 1×106 of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q
Figure 2. Plant RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q
Figure 3. Animal tissue RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q
