AccuPrep® Universal RNA Extraction Kit

AccuPrep® Universal RNA Extraction Kit allows rapid extraction of RNA from cultured cells, mammalian tissues, and plant tissues. The isolated RNA can be used for various experiments such as RT-PCR, quantitative real time RT-PCR, microarray analysis, etc.

Spin column type

$170.00
In stock
SKU
K-3140-CFG

Features and Benefits

  • Flexible sample choice
    Isolation of RNA from a wide variety of samples such as cultured cells, animal tissues, plant tissues, etc.

  • High binding capacity
    Up to 120 µg using silica based RNA binding columns

  • Minimized contamination
    Columns Individually sterilized and packaged 

  • Convenience
    Ethanol already added to the Wash Buffer

Application

• cDNA synthesis

• RT-PCR

• Quantative real-time RT-PCR

• Northern blot analysis

 Specifications

Column loading volume 800 μl
Max binding capacity ~ 120  μg
Elution volume 30  μl - 100  μl
Preparation time ~ 20 min

Sample Amount RNA Yield Purity
Cultured cells 104 – 108 cells 15 – 20 μg A260/280 > 2.0
A260/230 > 1.7
Mammalian tissue 25 – 50 mg 10 - 60 μg
Plant tissue 100 mg 30 - 60 μg

* Differences in the quantified value may occur depending on the types of samples.

 Components

Components K-3141
(50 reaction)
K-3140
(100 reaction)
RB Buffer (RNA Binding) 30 ml x 1 ea 60 ml x 1 ea
RWA1 Buffer (1st Washing) 40 ml x 1 ea 80 ml x 1 ea
RWA2 Buffer (2nd Washing) 70 ml x 1 ea 70 ml x 2 ea
ER Buffer (Elution) 10 ml x 1 ea 20 ml x 1 ea
AccuPrep® Binding Column-Ⅲ 50 ea 100 ea
1.5 ml Tube (for collection) 50 ea 100 ea
User's Guide 1 ea 1 ea

 Experimental data


Figure 1. Cultured cell RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 1×106 of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 2. Plant RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 3. Animal tissue RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q

Manuals

Manual_AccuPrep® Universal RNA Extraction Kit

 

Brochure

AccuPrep® nucleic acid extraction kit series

 

MSDS

• MSDS AccuPrep® Universal RNA Extraction Kit

 

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

Cat. No. Product Description Price
K-3140 AccuPrep® Universal RNA Extraction Kit, 100 reactions  $300.00
K-3141 AccuPrep® Universal RNA Extraction Kit, 50 reactions $170.00
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