AccuPrep® Nano-Plus Plasmid Midi Extraction kit (25 reactions)

▶ Specifications

▶ Components

Component	K-3122 (25 reaction)
PNA1 Buffer (Resuspension)	100 ml X 1 ea
P2 Buffer (Lysis)	110 ml X 1 ea
PA3 Buffer (Neutralization)	100 ml X 1 ea
W2 Buffer (Washing)	200 ml X 3 ea
EA Buffer (Elution)	60 ml X 1 ea
RNase A Powder, lyophilized	12 mg X 1 ea
Clearing Syringe Filter (30 ml)	25 ea
DNA binding column tube	25 ea
One Page Protocol	1 ea

▶ Procedure

▶ Experimental data

Figure 1. Reduced Total Prep. Time
The centrifugation time for obtaining cleared lysate (the solution containing plasmid DNA) by Buffer ① (Resuspension buffer) of this product has been shortened from traditional time of 20 minutes to just 5 minutes thanks to the nanoparticles inside the solution. AccuPrep® Nano-Plus Plasmid Midi Extraction Kit can extract plasmid DNA on midi scale (up to 75-100 ug) only within 40 minutes (30 minutes if used with air-pressure method).  

B; AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit
P, Q; Competitors

Figure 2. Improved Prep. Yield
AccuPrep® Nano-Plus Mini Extraction Kit is capable of providing high yields of both high- and low-copy number of highly pure plasmid DNA.

Figure 3. Restriction enzyme digestion test

 • Plasmid DNA isolated with AccuPrep® Nano-Plus Plasmid Mini Extraction Kit and a competitors' kit was verified and compared using agarose gel electrophoresis after restriction enzyme digestions.

pBS - pBluescript SK(+) with XbaI
pBI121 - pBI121 with PstI
Lane M - Molecular Marker(λ-DNA/ HindIII+EcoRI, Cat. No. D-1070, Bioneer)
Lane B - AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit
Lane Q, P - competitors

• Plasmid DNA isolated using AccuPrep® Nano-Plus Plasmid Mini Extraction Kit was verfied with agarose gel electrophoresis after digesting with various restriction enzymes.

Left - pBluescript SK(+)
Right - pBI121
Lane M - Molecular Marker(Cat. No. D-1040, Bioneer)

◈ Manual

• Manual_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit

• One page protocol_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit

◈ MSDS

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_PNA1 Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_P2 Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_PA3 Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_PB Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_W2 Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_EA Buffer

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_RNase A

• MSDS_AccuPrep^® Nano-Plus Plasmid Midi Extraction Kit_BST Solution

◈ Brochure

• AccuPrep^® nucleic acid extraction kit series

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate