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T4 DNA Ligase from Bioneer

for ligation of DNA, TA cloning, and other recombinant DNA applications

Overview
Technical/Specs
Literature/Support
Ordering Information

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will ligate DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase is isolated from a recombinant E.coli strain.


Features and Benefits

 High Speed: DNA ligations in 5 minutes for cohesive end and in 10 minutes for blunt end DNA at 25°C
 Flexibility: Suitable for all common DNA ligations
 Reproducibility: Batch manufacturing under strict ISO 9001 quality control system