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Pfu DNA Polymerase from Bioneer

Novel enzyme for high fidelity PCR with DNA proofreading

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Pfu DNA polymerase is a thermostable DNA polymerase isolated from Pyrococcus furiosus Vc1. It catalyzes the DNA-dependent polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction and exhibits 3’ → 5’ exonuclease (proof reading) activity.  Pfu DNA polymerase is the ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by PCR reaction.  It can apply to cloning, gene expression, site-directed mutagenesis and etc.


Features and Benefits

 High fidelity PCR: 3’ → 5’exonuclease(proofreading) activity
 Thermostability: Retaining 94-99% of its thermostable activity after 1 hour at 95°C.
 Terminal Transferase Activity: Devoid of terminal transferase activity and generates blunt-ended PCR products.