HotStart DNA Polymerase from Bioneer
Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated HotStart PCR method that, unlike most other
HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step.
Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures
above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase.
This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and
results in improved PCR specificity.