Fluorescence spectroscopy is a valuable tool for detecting fluorescence levels of biological molecules with fluorophores (dyes)
such as Marina Blue, Pacific Blue, Oregon Greens, CyTMdyes, AMCA, Bodipy derivatives, Fluorescein derivatives,
FAM, JOE, TET, HEX, VIC, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Cy5, Cy5.5, etc. Quenchers are generally used for quenching fluorescent
signals from the fluorescent dyes. These quenching effects have been widely using for detecting the level of the molecule which is of
interest in cells or in samples. Previously, BHQ quenchers (BHQ1, BHQ2) were commonly used because they have a high potency of
quenching activity. However BHQ1 and BHQ2 are only active in a narrow range of wavelength (BHQ1; ca 400-570 nm, BHQ2; ca 560-700 nm).
In this regard, Bioneer has developed a new, more widely applicable quenching molecule called EBQ (Excellent Bioneer Quencher),
which has enhanced quenching effects over a wider range of wavelengths (400-700 nm) than BHQs . Now experience the benefits of
EBQ – wide wavelength coverage and superior quality, while reducing the cost of your fluorescence spectroscopy experiment!
Figure 1. EBQ effectively quenched most commonly used reporter-dyes (Absorption spectrum of EBQ with the emission spectra of reporter-dyes).
Features and Benefits
| Broadrange wavelength
|A wide absorbance range of 400 ~ 700 nm for effective quenching of emission signal from dyes. You can unify
quenchers into EBQ (Maximum absorption occurred at 570 nm).
| High Stability:
||A structurally stable as a quencher against changes in temperature or pH
| Variety option of selectable dyes:
||EBQ effectively quenches most commonly used reporter-dyes with emission of 400-700 nm wavelength range such as Marina Blue,
Pacific Blue, Oregon Greens, CyTMdyes, AMCA, Bodipy derivatives, Fluorescein derivatives, FAM, JOE, TET, HEX, VIC, Cy3, NED, TAMRA, Cy3.5, ROX, Texas Red, Cy5, Cy5.5, etc.
| Fast and reliable Customer Service:
||Quick turnaround time: 4-5days from order to bench in most cases.
| Competitive pricing:
||Great value for your research dollar.