Features of CPG
Controlled Pore Glass (CPG) is the gold standard for solid-phase oligonucleotide synthesis,
with features such as high surface area, tightly controlled pore size and chemical inertness.
A variety of CPGs for oligonucleotide synthesis are available and can be purchased for standard synthesis sizes or for long-mer synthesis.
3’-Amino-CPG is used to place a 3’-amine end into oligonucloetides. It can be used to bind with other target materials with
a NHS-ester moiety.
3′-TAMRA-CPG is a fluorescent compound with λ excitation and λ emission of 565 nm and 580 nm, respectively, which has
been shown to be a useful quencher for dual-labeled oligo probes because of its spectral overlap with other fluorophores.
3'-Dabcyl-CPG is used to prepare probes with the quencher blocking the 3'-terminus.
The 3'-C3-disulfide CPG is used to introduce 3'-thiol linkages with three and six atom spacers into oligonucleotides.
This CPG is used to introduce a dithiol group at the 3'-terminus.
3’-Fluorescein CPG is used to label fluorescein at the 3’ end of oligonucleotides.