AccuPower® Plus DualStar™ qPCR PreMix (with UDG) is the most appropriate kit for providing
accurate data for multiple probes quickly from your various samples. All reaction components required for real-time PCR,
except for the target-specific primers and probes, are provided in each tube in a lyophilized "PreMix" form.
Just add primers and fluorescent dye-labeled probe for your target genes into Plus DualStar™ qPCR PreMix (with UDG)
tube to obtain reproducible results. This product helps you monitor the progress of your gene amplification in every PCR cycle
with primers and fluorescent probe you add. In addition, AccuPower® Plus DualStar™ qPCR PreMix (with UDG)
can be stored for two years in -20˚C freezer, and Bioneer provides several different types of formats of tubes
and plates to satisfy your needs depending on your real-time PCR machine.
Features and Benefits
Prevention of carryover contamination:
UDG and dUTP in the PreMix or in the Master Mix prevent the re-amplification of carryover PCR products between reactions.
The dUTP ensures that any amplified DNA will contain uracil rather than thymine. And UDG removes uracil residues
from single- or double-stranded DNA, which prevents uracil-containing DNA from serving as template in future PCRs.
In PCR with AccuPower® Plus DualStar™ qPCR PreMix (with UDG) the UDG incubation step
at (37°C for 2 min) destroys any uracil-contaning DNA fragments from previous reactions.
UDG is inactivated by the high temperatures during normal PCR cycling, thereby allowing the amplification
of genuine target sequences.
Figure 1. Prevention of carryover contamination by UDG enzyme and dUTP.
It shows wide range of copies over 8 logs from 10 to 108 copies.
A Non-specific reaction is dramatically eliminated by using Enzyme-mediated HotStart Technology (patented).
Universality of target gene:
You can always achieve great real-time PCR result regardless of various types of template DNA - gDNA, cDNA, high GC-enriched template and more.
In this kit, the product format provided is either freeze-dried PreMix or Master Mix. Both include everything required for the reaction
in an individual tube. Therefore, the users need to add only template RNA, primers and DEPC-treated water,
then it is ready to run the real-time PCR. Can be used with a wide variety of qPCR systems.
Under ISO 9001 Quality Assurance System, our mass-produced product AccuPower® Plus DualStar™ qPCR PreMix
or 2X Master Mix (with UDG) provides consistency for gene amplification.