AccuPower® RocketScript™ RT PreMix contains BIoneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript™.
Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure.
RocketScript™ has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA.
The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction,
including RTase, RNase inhibitor and buffer components.
Just add template RNA, primers and water, and the RT reaction is ready to go.
Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript™ shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
Features and Benefits
RocketScript™ is able to perform reverse transcription reactions throughout a wide range of temperatures from 42°C
RocketScript™ has enhanced performance to handle both high and low input RNA concentrations as well as short and long
RT target sizes.
The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse
transcription results in the tube.
Bioneer's strict quality controlled production system ensures that your results will be reproducible experiment after
Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.
Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer
RNase, DNase and Proteinase-free:
Ensures the integrity of your samples.
Broad range of working temperatures:
For RNAs that are G:C rich or significant secondary structure
Stable for 2 years at -20°C:
Long shelf life